Description	Ready-to-use PVDF membrane with 11 human tissue lysates (20 ug total protein per lane).
Background	INSTA-Blot͐ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot͐ Human Tissues NBP2-30113 is a simple and fast solution for screening proteins from various human tissues. We"ve streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-Blot͐,... immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop. Show More
PreparationMethod	INSTA-Blot͐ Human Tissues NBP2-30113 contains denatured proteins from human lysates loaded at 20 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolutionby SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot͐ is sold ready-to-use in your western blot protocol

Application Notes

Tissues are from donors with no known disease. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol). Use in western blot reported in scientific literature (PMID: 24704276).

Publications

ReadPublications using NBP2-30113 in the following applications: IHC 1 publication WB 7 publications

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