Additional Information	Newer 145 aa Version is Available!
Details of Functionality	Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.1-0.6 ng/mL.The specific activity of Recombinant Human FGF basic/FGF2/bFGF is approximately800IU/μg, which is calibrated against recombinant human FGF basic/FGF2basic WHO International Standard (NIBSC code: 90/712).
Source	E. coli-derived human FGF basic/FGF2/bFGF proteinPro143-Ser288, with an N-terminal Ala
Accession #	P09038
N-terminal Sequence	Ala-Pro143
Protein/Peptide Type	Recombinant Proteins
Gene	FGF2
Purity	>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note	<0.10 eu="" per="" 1="" μg="" of="" the="" protein="" by="" the="" lal="">

Theoretical MW	16.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE	17 kDa, reducing conditions
Publications	ReadPublications using 233-FB in the following applications: Binding Assay 1 publication Bioassay 335 publications Cell Culture 14 publications Differentiation 2 publications Dififerentiation 1 publication ELISA (Standard) 1 publication In Vivo 9 publications Tissue Culture 1 publication neural differentiation 1 publication

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