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商品描述
| Kitsize | 12x8 |
| Method | ELISA |
| Incubationtime | 1x18h,1x1h |
| Standardrange | 15.6-1000pg/mL |
| Specimen/Volumes | 100µLtissueculturemedia,serum,plasmaorsaliva,milksamples |
| Substrate/isotope | PNPP405nm |
| RegulatoryStatus: | Forresearchuseonly |
Detailsfor:OxytocinELISA
IBL我们的液体处理专业知识可实现高度复杂的分子诊断技术的可靠自动化，例如细胞遗传学（核型分析和FISH）和分子细胞遗传学（aCGH /阵列比较基因组杂交），其应用范围从代谢疾病和癌症到生殖遗传学和移植药物。该产品组合涵盖了细胞遗传学和分子细胞遗传学工作流程的关键阶段，从标准化工作站，经过验证的应用程序和专用用户界面，到与Tecan Labwerx合作开发的高度定制的平台。 TheOxytocinELISAkitisacompetitiveimmunoassayforthequantitativedeterminationofOxytocininsamples.ThekitusesapolyclonalantibodytoOxytocintobind,inacompetitivemanner,theOxytocininthestandardorsampleoranalkalinephosphatasemoleculewhichhasOxytocincovalentlyattachedtoit.
Afterasimultaneousincubationat4°Ctheexcessreagentsarewashedawayandsubstrateisadded.Afterashortincubationtimetheenzymereactionisstoppedandtheyellowcolorgeneratedreadonamicroplatereaderat405nm.TheintensityoftheboundyellowcolorisinverselyproportionaltotheconcentrationofOxytocinineitherstandardsorsamples.ThemeasuredopticaldensityisusedtocalculatetheconcentrationofOxytocin.
Oxytocinisaneurohypophysialpeptidewhichisproducedintheparaventricularnucleiofthehypothalamusandstoredintheposteriorpituitary.Themoleculeconsistsofnineaminoacidslinkedwitha[1-6]disulfidebondandasemi-flexIBLecarboxyamidatedtail.Ahormoneoncethoughttobelimitedtofemalesmoothmusclereproductivephysiology,morecurrentfindingshavedeterminedthatoxytocinalsofunctionsasaneurotransmitter,maybeinvolvedinneuropsychiatricdisorders,social/sexualbehaviorandisimportantinmalereproductivephysiology.Oxytocinandtherelatedneurohypophysialpeptide,Arg8-Vasopressin,maintainrenalwaterandsodiumbalance.
Oxytocin:H-Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Leu-Gly-NH2
Highlyconservedacrossspeciesboundaries,oxytocin-likeneurohypophysialpeptidesaresubstitutedprimarilyatresidues4and/or8.Intheoxytocin-likepeptide,mesotocin,acommonpeptidefoundinsomefishes,reptiles,amphibians,marsupialsandnonmammaliantetrapods,theleucineatresidue8issubstitutedforisoleucine8.Actinginclassicalendocrinefashion,Oxytocinelicitsregulatoryeffectsbybindingspecificcellsurfacereceptorswhichinturninitiateasecondaryintracellularresponsecascadeviaaphosphoinositidesignalingpathway.
DistributedbyIBLInternational
ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.Afterasimultaneousincubationat4°Ctheexcessreagentsarewashedawayandsubstrateisadded.Afterashortincubationtimetheenzymereactionisstoppedandtheyellowcolorgeneratedreadonamicroplatereaderat405nm.TheintensityoftheboundyellowcolorisinverselyproportionaltotheconcentrationofOxytocinineitherstandardsorsamples.ThemeasuredopticaldensityisusedtocalculatetheconcentrationofOxytocin.
Oxytocinisaneurohypophysialpeptidewhichisproducedintheparaventricularnucleiofthehypothalamusandstoredintheposteriorpituitary.Themoleculeconsistsofnineaminoacidslinkedwitha[1-6]disulfidebondandasemi-flexIBLecarboxyamidatedtail.Ahormoneoncethoughttobelimitedtofemalesmoothmusclereproductivephysiology,morecurrentfindingshavedeterminedthatoxytocinalsofunctionsasaneurotransmitter,maybeinvolvedinneuropsychiatricdisorders,social/sexualbehaviorandisimportantinmalereproductivephysiology.Oxytocinandtherelatedneurohypophysialpeptide,Arg8-Vasopressin,maintainrenalwaterandsodiumbalance.
Oxytocin:H-Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Leu-Gly-NH2
Highlyconservedacrossspeciesboundaries,oxytocin-likeneurohypophysialpeptidesaresubstitutedprimarilyatresidues4and/or8.Intheoxytocin-likepeptide,mesotocin,acommonpeptidefoundinsomefishes,reptiles,amphibians,marsupialsandnonmammaliantetrapods,theleucineatresidue8issubstitutedforisoleucine8.Actinginclassicalendocrinefashion,Oxytocinelicitsregulatoryeffectsbybindingspecificcellsurfacereceptorswhichinturninitiateasecondaryintracellularresponsecascadeviaaphosphoinositidesignalingpathway.
DistributedbyIBLInternational
