| Kitsize | 12x8 |
| Method | ELISA |
| Incubationtime | 1x1h;1x30min |
| Standardrange | 0.37-30ng/mL |
| Specimen/Volumes | 20µLSerum |
| Substrate/isotope | TMB450nm |
| RegulatoryStatus: | EU:CE |
TheLC-MS/MSisourbenchmark
Dehydroepiandrosterone(DHEA)anditssulfateesterDHEA-Sarethemostabundantsexsteroidhormonesinhumans,providingalargeprecursorreservoirforthesynthesisofandrogensandestrogens.However,onlythenon-sulfated(free)DHEAshowsBIOLOGicalactivity.
IBLInternationaloffersahighlyspecificELISAfordeterminingserumDHEAlevels,whichwascalibratedagainstthereferencemethodLC-MS/MS.Theassay"sdistinguishingfeaturesare:
- ExceptionalconcordancetoLC-MS/MS:
IBLDHEAELISA=0.94xLC-MS/MS+0.13;r²=0.99;N=50 - Verygoodconcordancetothegoldstandard
ImmunotechRIA*):
IBLDHEAELISA=0.83xImmunotechRIA–0.37;r²=0.96;N=48
*)RIAwithextraction - Extraction-freeassay
- Adaptabletoopen-systemELISAanalyzers
- 2kitcontrols
MeasurementofserumDHEAisausefulMarkerofadrenalandrogensynthesis.Decreasedlevelsmayindicateadrenalinsufficiency.Elevatedlevelsarefoundinvariousdiseases,includingadrenalcorticalcarcinoma,tumorswithectopicACTHproduction,adrenalhirsutism,congenitaladrenalhyperplasia,andCushing"sdisease(pituitaryCushing"ssyndrome).
Moreover,ourDHEAELISA(RE52221)showsanexcellentagreementwiththegoldstandardassayRIAbyImmunotech. GeneralInformation Enzymeimmunoassayforthein-vitro-diagnosticquantitativedeterminationofDHEAinhumanserum.Dehydroepiandrosterone(DHEA;androstenolone;3ß-hydroxy-5-androsten-17-one)isaC19steroidproducedintheadrenalcortexand,toalesserextent,gonads.DHEAservesasaprecursorintestosteroneandestrogensynthesis.Duetothepresenceofa17-oxo(ratherthanhydroxyl)group,DHEAhasrelativelyweakandrogenicactivity,whichhasbeenestimatedat~10%thatoftestosterone.Howeverinneonates,peripubertalchildrenandinadultwomen,circulatingDHEAlevelsmaybeseveral-foldhigherthantestosteroneconcentrations,andrapidperipheraltissueconversiontomorepotentandrogens(androstenedioneandtestosterone)andestrogensmayoccur.Moreover,DHEAhasrelativelylowaffinityforsex-hormonebindingglobulin.ThesefactorsmayenhancethephysiologicbiopotencyofDHEA.ThephysiologicroleofDHEAhasnotbeenconclusivelydefined.Avarietyofinvitroeffects,includingantiproliferativeeffectsindifferentcelllinesandeffectsonenzyme-mediatedcellmetabolism,havebeenreported.InvivostudiessuggestthatDHEAmayaffectcholesterolandlipidmetabolism,insulinsensitivityandsecretionandimmunefunction.AbnormalDHEAlevelshavebeenreportedinschizophreniaandobesity.TherapeuticadmiNISTrationofDHEAhasbeenproposedforseveralconditions,includingobesityandcardiovasculardisease.SerumDHEAlevelsarerelativelyhighinthefetusandneonate,lowduringchildhood,andincreaseduringpuberty.IncreasedlevelsofDHEAduringadrenarchemaycontributetothedevelopmentofsecondarysexualhair.SerumDHEAlevelsprogressivelydeclineafterthethirddecadeoflife.NoconsistentchangesinserumDHEAlevelsoccurduringthemenstrualcycleorpregnancy;however,paritymaylowerserumDHEAlevelsinpremenopausalwomen.DHEAhasarapidmetabolicclearancerateascomparedtoitssulfatedconjugate,DHEA-S.Becauseofthis,serumDHEAlevelsare100-1000foldlowerthanDHEA-Slevels.SerumDHEAlevelsincreaseinresponsetoexogenousACTHadministration.MeasurementofserumDHEAisausefulmarkerofadrenalandrogensynthesis.Abnormallylowlevelsmayoccurinhypoadrenalism,andelevatedlevelsoccurinseveralconditions;includingvirilizingadrenaladenomaandcarcinoma,21-hydroxylaseand3ß-hydroxysteroiddehydrogenasedeficienciesandinsomecasesoffemalehirsutism.SinceverylittleDHEAisproducedbythegonads,measurementofDHEAlevelsmayaidinthelocalizationofandrogensourceinvirilizingconditions.
