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商品描述
| Kitsize | 12x8 |
| Method | ELISA |
| Incubationtime | 1x2h,1x30min,1x15min |
| Standardrange | 5.3-133.0ng/ml |
| Specimen/Volumes | 50µLSerum |
| Substrate/isotope | TMB450nm |
| RegulatoryStatus: | Forresearchuseonly |
Detailsfor:25-hydroxy-VitaminDtotal(rat)ELISA
IBL我们的液体处理专业知识可实现高度复杂的分子诊断技术的可靠自动化，例如细胞遗传学（核型分析和FISH）和分子细胞遗传学（aCGH /阵列比较基因组杂交），其应用范围从代谢疾病和癌症到生殖遗传学和移植药物。该产品组合涵盖了细胞遗传学和分子细胞遗传学工作流程的关键阶段，从标准化工作站，经过验证的应用程序和专用用户界面，到与Tecan Labwerx合作开发的高度定制的平台。 Immunoenzymetricassayforthequantitativemeasurementof25-hydroxyvitaminD2andD3(25OH-D2and25OH-D3)inratserum.
VitaminDisthegenerictermusedtodesignateVitaminD2orergocalciferolandVitaminD3orcholecalciferol.
Inthelivermainly,VitaminD3ismetabolisedinto25-HydroxyvitaminD3(25OHD3)whichisthemainformofVitaminDcirculatinginthebody.
25OHD3isaprecursorforotherVitaminDmetabolitesandhasalsoalimitedactivitybyitself.
Themostactivederivativeis1,25-hydroxyvitaminD3,producedinthekidney(orplacenta)by1-hydroxylationof25OHD3.
25OHVitaminDstimulatestheintestinalabsorptionofbothcalciumandphosphorusandalsoboneresorptionandmineralisation.Ratshavebeenusedasexperimentalmodeltoinvestigatetheeffectof25OHVitaminDsupplementsonfracturehealing.
25OHVitaminDmightalsobeactiveinothertissuesresponsIBLeforcalciumtransport(placenta,kidney,mammarygland…)andendocrinegland(parathyroidglands,betacells…).
VitaminD3andVitaminD2arealsoavailablebyingestionthroughfoodordietarysupplementation.
AsVitaminD2ismetabolisedinasimilarwaytoVitaminD3,bothcontributetotheoverallVitaminDstatusofanindividual.
StudiesonratshavebeenconductedtocomparemetabolismoforalVitaminD2andoralVitaminD3.(6)andtodefinetheeffectofVitaminD3intakeoncalciummetabolism.
InfluenceofvitaminDdeficiencyduringintrauterineandearlylifeonbloodpressurehasbeensuitedinaratmodel.InstudiesonratPTH(parathyroidhormone),25OHvitaminDismeasuredinratserumtodetermineitsinfluenceonPTHsecretion.
ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.VitaminDisthegenerictermusedtodesignateVitaminD2orergocalciferolandVitaminD3orcholecalciferol.
Inthelivermainly,VitaminD3ismetabolisedinto25-HydroxyvitaminD3(25OHD3)whichisthemainformofVitaminDcirculatinginthebody.
25OHD3isaprecursorforotherVitaminDmetabolitesandhasalsoalimitedactivitybyitself.
Themostactivederivativeis1,25-hydroxyvitaminD3,producedinthekidney(orplacenta)by1-hydroxylationof25OHD3.
25OHVitaminDstimulatestheintestinalabsorptionofbothcalciumandphosphorusandalsoboneresorptionandmineralisation.Ratshavebeenusedasexperimentalmodeltoinvestigatetheeffectof25OHVitaminDsupplementsonfracturehealing.
25OHVitaminDmightalsobeactiveinothertissuesresponsIBLeforcalciumtransport(placenta,kidney,mammarygland…)andendocrinegland(parathyroidglands,betacells…).
VitaminD3andVitaminD2arealsoavailablebyingestionthroughfoodordietarysupplementation.
AsVitaminD2ismetabolisedinasimilarwaytoVitaminD3,bothcontributetotheoverallVitaminDstatusofanindividual.
StudiesonratshavebeenconductedtocomparemetabolismoforalVitaminD2andoralVitaminD3.(6)andtodefinetheeffectofVitaminD3intakeoncalciummetabolism.
InfluenceofvitaminDdeficiencyduringintrauterineandearlylifeonbloodpressurehasbeensuitedinaratmodel.InstudiesonratPTH(parathyroidhormone),25OHvitaminDismeasuredinratserumtodetermineitsinfluenceonPTHsecretion.
