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Glen research/Glen Gel-Pak™ 1.0 Desalting Column/1kit/61-5010-50

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¥9000.00
货号:61-5010-50
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品牌:Glen research
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Guide to Glen GelPak Purification

Glen Report 22.25: New Products - Glen Gel-Pak™ DNA/RNA Desalting and 1000Å Glen UnySupport™ Frits


Description

The principle of the Glen Research gel filtration column, Glen Gel-Pak™ , is based on size exclusion chromatography that separates molecules according to the hydrodynamic volume of the molecule in aqueous solutions. In gel filtration, the mobile phase for size exclusion is an aqueous solution and the stationary phase is a porous resin. The pores of the resin are sized such that they allow small molecules to enter the pores, yet exclude larger molecules from the pores. The small molecules, such as salts and hydrolyzed protecting groups, diffuse into the pores of the resin and move slowly through the column. The larger molecules, such as DNA or proteins, are excluded from the pores and move quickly through the column. The end result is that the larger molecules elute first in the column void volume while the small molecules are still flowing through The resin of the column. Glen Gel-Pak columns are ideal for desalting and reaction clean up. They can be used for removal of the ammonium hydroxide deprotection solution and hydrolyzed protecting groups after deprotection. The columns can also be used for the clean up of NHS-labelling reactions to separate the labelled oligo and unlabelled oligo from the unreacted NHS ester, the hydrolyzed label, and n-hydroxysuccinimide, thereby greatly simplifying the downstream purification steps.There are many benefits to Glen Gel-Pak columns:Versatility:•Ability to directly desalt oligonucleotides deprotected in either 30% ammonium hydroxide OR 50:50 ammonium hydroxide/40% aqueous methylamine (AMA)•Easily exchange buffers•Simple clean-up of labelling reactions•Mild method for purification from salts and solvents such as DMSO and DMFCapacity:Multiple column sizes (0.2 mL, 1.0 mL and 2.5 mL) are available to match synthesis scaleAbility to efficiently desalt short and long oligos at different scales using the same protocolSuitable for oligos >10mer in length

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Specifications
StorageControlled room temperature


Glen research表观遗传学是生物学和癌症研究中发展最快的领域之一。虽然基本的遗传密码定义了合成哪些蛋白质和基因产物,但表观遗传控制定义了它们何时何地表达。基因表达的这种动态控制对于X染色体失活,胚胎发生,细胞分化至关重要,并且似乎是记忆形成和突触可塑性的组成部分。在2009年,两份报告1,2  中所述5-羟甲基-2'-脱氧胞苷的发现(HMDC),浦肯野神经元和胚胎干细胞的新颖的DC修饰。后来,第三份报告发现这种修饰在与较高认知功能相关的脑组织中高度丰富。3 dC修饰是通过α-酮戊二酸依赖性十一种11易位(TET)酶的作用产生的,该酶将5-Me-dC氧化为hmdC。这一发现激发了关于可能通过例如碱基切除修复(BER)借助专门的DNA糖基化酶发生的活性脱甲基途径的讨论。或者,可以设想一种方法,其中将hmdC的羟甲基进一步氧化为5-甲酰基-dC(fdC)或5-羧基-dC(cadC),然后消除甲酸或二氧化碳4,5。Glen Research自成立以来就一直为这项研究提供支持,为合成包含所有新dC衍生物-hmdC,fdC和cadC的寡核苷酸提供了基础。第一代hmdC亚磷酰胺已被广泛接受,但需要相当苛刻的脱保护条件。因此,介绍了由Carell和同事开发的与UltraMild脱保护兼容的第二代构建基(5-Hydroxymethyl-dC II)。6  5-甲酰基-dC III旨在满足制备包含所有甲基化变体的寡核苷酸的所有要求。