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Glen research/Universal Support III PS/1kit/26-5010-01

价格
¥320.00
货号:26-5010-01
浏览量:109
品牌:Glen research
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Technical Documents

Safety Data Sheet

UNIVERSAL SUPPORT II AND III

Glen Report 11.15: Update - Universal Supports, Q-supports

Glen Report 23.29: New Products - Bulk Polystyrene Supports

Glen Report 20.110: Improving Universal Support II for Oligonucleotide Synthesis

Glen Report 10.15: Universal Support Replaces Individual Columns

Glen Report 31.25: Technical Brief – Universal Support IIIPS Cleavage and Dephosphorylation


Description

Some of our more popular minor base and modifier supports are available on polystyrene in columns fully compatible with the Applied Biosystems 3900 synthesizer. These include our popular Universal Support III, which will allow DNA, RNA or LNA oligos to be produced on the 3900 with ANY base at the 3" terminus. At the same time, we are offering 1 μmole columns of Universal Support III for the 3900 instrument. Structures and more complete descriptions are found in the relevant catalog sections for each item. AB 3900 columns can be prepared with virtually any of the CPG supports in this catalog.It is no longer necessary to adjust the flow using our AB 3900 CPG columns, as noted in the box to the right.Modified CPG columns are only available in 200 nmole size -simple add ‘A’ to the regular catalog number to order.

Details

Usage

  • Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
  • Deprotection: See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_Universal_Support_III.pdf).|Note:This product is not compatible with cyanoethyl protecting group removal protocols using hindered bases such as DEA or DBU. Removal of the cyanoethyl groups prior to cleavage will prevent the release of the oligonucleotide from the support.
Specifications
StorageRefrigerated storage, maximum of 2-8°C, dry

Intellectual Property

This product is covered by US Patent Nos.:6,770,754 and 7,491,817 and European PatentNos.: 1404695 and 2248820.


Glen research表观遗传学是生物学和癌症研究中发展最快的领域之一。虽然基本的遗传密码定义了合成哪些蛋白质和基因产物,但表观遗传控制定义了它们何时何地表达。基因表达的这种动态控制对于X染色体失活,胚胎发生,细胞分化至关重要,并且似乎是记忆形成和突触可塑性的组成部分。在2009年,两份报告1,2  中所述5-羟甲基-2'-脱氧胞苷的发现(HMDC),浦肯野神经元和胚胎干细胞的新颖的DC修饰。后来,第三份报告发现这种修饰在与较高认知功能相关的脑组织中高度丰富。3 dC修饰是通过α-酮戊二酸依赖性十一种11易位(TET)酶的作用产生的,该酶将5-Me-dC氧化为hmdC。这一发现激发了关于可能通过例如碱基切除修复(BER)借助专门的DNA糖基化酶发生的活性脱甲基途径的讨论。或者,可以设想一种方法,其中将hmdC的羟甲基进一步氧化为5-甲酰基-dC(fdC)或5-羧基-dC(cadC),然后消除甲酸或二氧化碳4,5。Glen Research自成立以来就一直为这项研究提供支持,为合成包含所有新dC衍生物-hmdC,fdC和cadC的寡核苷酸提供了基础。第一代hmdC亚磷酰胺已被广泛接受,但需要相当苛刻的脱保护条件。因此,介绍了由Carell和同事开发的与UltraMild脱保护兼容的第二代构建基(5-Hydroxymethyl-dC II)。6  5-甲酰基-dC III旨在满足制备包含所有甲基化变体的寡核苷酸的所有要求。