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商品描述
| ProductName | Dr1(NC2b19kDa) |
| Size | 10,000U |
| Description | Dr1isageneralnegativeregulatorofclassIIandclassIIIgeneexpression(1,2).ItbindstothebasicrepeatdomainofTBPonpromoterDNAandcanpreventtheRNApolymeraseIIholoenzyme,oritsTFIIBand/orTFIIAsubunits,fromassemblingintoaninitiationcomplex(3,4).Dr1isphosphorylatedinvivoandthismodificationaffectsitsinteractionwithTBP(1).Inaddition,Dr1interactswiththehyperphosphorylatedformofPolIIandwiththerepressiondomainoftheAREB6repressor(5,6).Dr1formsaheterodimercomplexwithDRAP1throughitshistonefolddomain(7).MorerecentlyitwasdiscoveredthatDr1-DRAP1isabi-functionalbasaltranscriptionfactorthatdifferentiallyregulatesgenetranscriptionthroughDPE(downstreampromoterelements)orTATAboxmotifs(8).ItcanstimulatetranscriptioninvitrofromDrosophilapromoterscontainingDPEs,whereasitrepressestranscriptionfromTATA-containingpromoters(8). |
| Applications |
|
| Source | E.coli |
| FusionTag | His |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogramofpurifiedprotein.1unitissufficientforagelmobilityshiftassayina20µlreaction;100unitsaresufficientforprotein-proteininteractionassays |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 1810 |
| Accession# | NM_001938 |
| GeneName | down-regulatoroftranscription1,TBP-binding(negativecofactor2) |
| GeneSymbol | DR1 |
| GeneSynonyms | NC2;NC2-BETA |
| MW | 20.6kDa |
| ProteinSequence | MASSSGNDDDLTIPRAAINKMIKETLPNVRVANDARELVVNCCTEFIHLISSEANEICNK SEKKTISPEHVIQALESLGFGSYISEVKEVLQECKTVALKRRKASSRLENLGIPEEELLR QQQELFAKARQQQAELAQQEWLQMQQAAQQAQLAAASASASNQAGSSQDEEDDDDI |
| StorageBuffer | 20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Inostroza,J.A.,etal.,(1992)Cell70,477-489 2.White,R.J.,etal.,(1994)Science266,448-451 3.Mermelstein,F.,etal.,(1996)GenesDev.10,1033-1048 4.Kim,T.K.,etal.,(1995)J.Biol.Chem.270,10976-10981 5.Castano,E.,etal.,(2000)Proc.Natl.Acad.Sci.USA97,7184-7189 6.Ikeda,K.,etal.,(1998)Mol.Cell.Biol.18,10-18 7.Goppelt,A.,etal.,(1996)EMBOJ.15,3105-3116 8.Willy,P.J.,etal.,(2000)Science290,982-9858 9.Denkoetal.,(2003)J.ofBio.Chem.,278:5744-5749 |
