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蚂蚁淘在线 / 品牌中心 / Excellgen / Excellgen/Pseudouridine Modified 5 Anti-Reverse Cap Analog (ARCA) capped FLP recombinase mRNA, NLS-hFLP/EG-1044/100 μg

Excellgen/Pseudouridine Modified 5 Anti-Reverse Cap Analog (ARCA) capped FLP recombinase mRNA, NLS-hFLP/EG-1044/100 μg

价格
￥39000.00
货号：EG-1044
浏览量：127
品牌：Excellgen
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商品描述
Description

FLPrecombinasecatalyzesthesite-specificrecombinationofDNAbetweenFRTsites.Ithasbeenwidelyusedforgeneratingconditionalknockoutmice,mediatedbyFLP/FRTsystem.

CodonoptimizedhFLPisahuman-codonoptimizedThermo-stablemutantformofFLP,withenhancedrecombinaseactivitybya4-10foldhigherthanFLPat37°C.

RecentstudiesdemonstatedthatpseudouridinemodifiedmRNAsignaficantlyreducePKRactivationincells,therebyenhancestranslationoftheencodedproteinafterbeingdeliveredintoculturecellsorinjectedintoanimals.

Pseudouridinemodified5'Anti-ReverseCapAnalog(ARCA)cappedNLS-hFLPrecombinase(humancodon-optimizedNLS-hFLP)mRNAhasbeentestedforFRTrecombinationinFRT-RFP-Stop-FRT-GFPreportercells(e.g.,HEK293,Hela,andNIH3T3),andprimarycells.

Applications

  • exvivoFRTrecombination.
  • invivoFRTrecombination.
Size

5µg

TransfectionProtocols

1.InvitrogenRNAiMAXmRNATransfectionProtocol
2.MirusBioTransIT-mRNAmRNATransfectionProtocol

Storage-20°Cor-80°C
Shippingdryice
References
  1. Kariko,K.etal.IncorporationofpseudouridineintomRNAyieldssuperiornonimmunogenicvectorwithincreasedtranslationalcapacityandBIOLOGicalstABIlity.Mol.Ther.16,1833–1840(2008).
  2. Kariko,K.,Muramatsu,H.,Keller,J.M.&Weissman,D.Increasederythropoiesisinmiceinjectedwithsubmicrogramquantitiesofpseudouridine-containingmRNAencodingerythropoietin.Mol.Ther.20,948–953(2012).
  3. Kormann,M.S.etal.ExpressionoftherapeuticproteinsafterdeliveryofchemicallymodifiedmRNAinmice.Nat.Biotechnol.29,154–157(2011).
  4. Warren,L.etal.HighlyefficientreprogrammingtopluripotencyanddirecteddifferentiationofhumancellswithsyntheticmodifiedmRNA.CellStemCell7,618–630(2010).
Excellgen蛋白表达产品名称-目录 ＃价钱293Expresso™转染试剂EG-1089$ 98.00定制抗体生产和免疫测定开发EG-36从培养基中纯化IgEEG-1076-1$ 750.00MembraneExpress TM功能膜蛋白的病毒样颗粒EG-1038蛋白质表达和纯化服务EG-1076-8$ 1,500.00小规模表达测试和纯化方法开发EG-1081-3$ 1,250.00使用HEK 293细胞的瞬时蛋白质生产EG-1080$ 2,900.00  $ 1,950.00-$ 950.00使用细菌表达系统进行蛋白表达和纯化EG-1076$ 2,170.00使用杆状病毒表达系统进行蛋白质表达和纯化EG-1078$ 4,500.00使用Akta FPLC系统的定制蛋白质纯化服务EG-1082$ 1,000.00使用稳定的哺乳动物细胞系表达蛋白质EG-1081$ 9,500.00