Description | RecentstudiesdemonstatedthatpseudouridinemodifiedmRNAsignaficantlyreducePKRactivationincells,therebyenhancestranslationoftheencodedproteinafterbeingdeliveredintoculturecellsorinjectedintoanimals.Pseudouridinemodified5'Anti-ReverseCapAnalog(ARCA)cappedNLS-Crerecombinase(humancodon-optimizedNLS-Cremutant)mRNAhasbeentestedforloxPrecombinationinloxP-RFP-Stop-loxP-GFPreportercells(e.g.,HEK293,Hela,andNIH3T3),andprimarycells. |
Applications | - exvivoLoxPrecombination
- invivoLoxPrecombination
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TransfectionProtocols | 1.InvitrogenRNAiMAXmRNATransfectionProtocol 2.MirusBioTransIT-mRNAmRNATransfectionProtocol |
Storage | -20°Cor-80°C |
Shipping | dryice |
References | - Kariko,K.etal.IncorporationofpseudouridineintomRNAyieldssuperiornonimmunogenicvectorwithincreasedtranslationalcapacityandBIOLOGicalstABIlity.Mol.Ther.16,1833–1840(2008).
- Kariko,K.,Muramatsu,H.,Keller,J.M.&Weissman,D.Increasederythropoiesisinmiceinjectedwithsubmicrogramquantitiesofpseudouridine-containingmRNAencodingerythropoietin.Mol.Ther.20,948–953(2012).
- Kormann,M.S.etal.ExpressionoftherapeuticproteinsafterdeliveryofchemicallymodifiedmRNAinmice.Nat.Biotechnol.29,154–157(2011).
Warren,L.etal.HighlyefficientreprogrammingtopluripotencyanddirecteddifferentiationofhumancellswithsyntheticmodifiedmRNA.CellStemCell7,618–630(2010).
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