Description	YeastSUMOprotease(alsoknownasUlp),isahighlyactivieandrobustcysteinylproteaseencodedbytheUbl-specificprotease1genefromSaccharomycescerevisiae.ThereareasmanyassixSUMOproteasesinhuman(SENP1–3,SENP5–7).BothhumanandyeastSUMOproteasesrecognizethetertiarystructureoftheubiquitin-like(UBL)protein,i.e.,SUMO-1,SUMO-2andSUMO-3,ratherthanashortstretchofaminoacidsequence,consequently,theseSUMOproteaseswillnotcleavewithinthefusedproteinofinterest.TurboSUMOProteaseisamixtureofcorecatalyticproteasesofbothhumanSENP2andyeastUlp1SUMOproteins,thusSUMO-fusionproteinscanbedigestedbytheTurboSUMOProteaseateither~15°Covernightorat30°Cforafewhours.Afterdigestion,TurboSUMOProteasecanbeeasilyremovedfromthecleavagereactionusingNiagarosebeads
Application	RemovalofSUMOtagfromfusionproteins
Source	E.coliover-expressingtheC-terminalSENP2andUlp1catalyticproteasedomainsfromhumanandSaccharomycescerevisiae,respectively.
Purification	FPLC
Purity	>95%bySDS-PAGE
Components	10U/µlTurboSUMOProteasein50mMHepes,pH7.5,150mMNaCl,0.5mMDTT,50%glycerol
UnitDefinition	OneunitofSUMOProteaseisdefinedastheamountofenzymeneededtocleave85%of2µgofsubstrateproteinat30°Cor15°Cinonehour
RecommendedReactionCondition	SUMOProtease:Add1unitto2µgoftargetSUMOfusionprotein Buffer:50mMTris-HCl,pH8.0,0.1%Igepal(NP-40),150mMNaCl,1mMDTT Temperature:30°C(1~3hours),15°C(overnight) OptimalSaltConcentration:imidazole:<150mM;NaCl:<300mM
Storage	-20°C,orat–80°Cforlong-termstorage

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