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蚂蚁淘在线 / 品牌中心 / Excellgen / Excellgen/Turbo Human Rhinovirus 3C (HRV 3C) Protease, His-GST-HRV 3C/EG-1035/1,000 Units
商品描述
Description | RecombinantTurboHis-GST-HRV3CProteaseencodedbyhumanrhinovirus143Cgeneishighlyspeckficfor(therecognition"PreScission"sequenceLeu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro(LEVLFQ↓GP)andcleavesbetweentheGlnandGlyresidues.Itisengineeredasafusionproteinwithbothpolyhistidine(6xHis)andglutathione-S-transferase(GST)tags,whichfacilitateeasyremovaloftheproteaseaftercleavageusingeitherglutathione(GSH)ormetalchelatesresins.TurboHis-GSTHRV3CexhibitshighestactivityaroundneutralpHinavarietyofcommonlyusedbuffersattemperaturerangingfrom22to37℃.Italsoretainsrobustactivityat4℃,allowingremovalofpurificationtagsfromtargetproteinsatlowtemperature |
Applications | Removalofpurificationtagsfromfusionproteins |
Source | E.coliover-expressingsynthetic(enhanced)formofTEVNIaproteinasegene. |
Size | 1mg |
MW | 47.4kD |
SpecificActivity | >1Units/µg.1Uni~sofTuxboHir-GSTHRV3CProteasecleaves>95%of100µgoftargetproteinat4oCfor16hoursinRecommendedReactionBuffer:50mMTris-HCl,150mMNaCl,pH8.0 |
Storage | -20°C. |