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蚂蚁淘在线 / 品牌中心 / Excellgen / Excellgen/Turbo Human Rhinovirus 3C (HRV 3C) Protease, His-HRV 3C/EG-1048/10,000 Units
商品描述
| Description | RecombinantTurboHRV3CProteaseencodedbyhumanrhinovirus143Cgeneishighlyspecificfortherecognition"PreScission"sequenceLeu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro(LEVLFQ↓GP)andcleavesbetweentheGlnandGlyresidues.Ithasapolyhistidine(6xHis)tag,whichfacilitateseasyremovaloftheproteaseusingmetalchelateresinsaftercleavagereaction.TurboHRV3CexhibitshighestactivityaroundneutralpHinavarietyofcommonlyusedbuffersattemperaturerangingfrom22to37℃.Italsoretainsrobustactivityat4℃,allowingremovalofpurificationtagsfromtargetproteinsatlowtemperature. |
| Application | Removalofpurificationtagsfromfusionproteins |
| Source | E.coliover-expressingsynthetic(enhanced)formofTEVNIaproteinasegene. |
| Size | 1mg |
| Purification | FPLC |
| Purity | >95%bySDS-PAGE |
| SpecificActivity | >10Units/µg.1UnitofTurboTEVProteasecleaves>85%of3µgofcontrolsubstratein1hourat30oCinRecommendedReactionBuffer:50mMTris-HCl,150mMNaCl,pH8.0 |
| Storage | -20°C. |
