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商品描述
Description | TurboHis-GST-TEVproteaseisanenhancedformofthewild-typetobaccoetchvirus(TEV)NuclearInclusiona(NIa)protein,acysteineproteasethatrecognizesthecleavagesiteofE-Xaa-Xaa-Y-Xaa-Q-(G/S)(e.g.,ENLYFQG),andcleavesbetweenQandGorQandS.TurboHis-GST-TEVisnon-autoinactivable,hasrobustactivityat4oC,roomtemperatureor37oC,andhasmuchhighersequencespecificitythanfactorXa,thrombin,orenterokinase.TurboHis-GST-TEVproteasecanbeusedforremovingpurificationtagssuchasHis,GST,MBPfromfusionproteins.Afterdigestiontheenzymecanbeseparatedfromtargetproteinusingeithernickelorglutathioneagarosebeads. |
Source | E.coliover-expressingsynthetic(enhanced)formofTEVNIaproteinasegene. |
Size | 1mg,1ml |
SpecificActivity | >10Units/µg.1UnitofTurboHis-GST-TEVProteasecleaves>85%of3µgofcontrolsubstratein1hourat30oC. |
SDS-PAGE | |
Storage | -20°C. |