Description	T4DNAPolymerasecatalyzestheextensionofaprimedDNAtemplate(inthepresenceoftemplateandprimer)inthe5′→3′direction.ThisD219AmutantT4DNAPolymerasedoesnotpossesseither3'->5’(proofreADIng)and5′→3′exonucleaseactivities.
Applications	Randommutagenesis
Source	ArecombinantE.colistraincarryingaclonedgene43(D219Amutant)ofbacteriophageT4.
UnitDefinition	Oneunitisdefinedastheamountofenzymethatwillincorporate10nmolofdNTPintoacid-precipitablematerialin30minutesat37°C.
Components	ExonucleaseDeficientT4DNAPolymerase:3,000U/mlin100mMKPO4,1.0mMDTT,0.1mMEDTA,50%glycerol,pH6.5@25°C 10XReactionBuffer:500mMNaCl,100mMTris-HCl,100mMMgCl2,10mMDTT,pH7.9@25°C
QualityControl	Theabsenceofendodeoxyribonucleasesconfirmedbyappropriatequalitytests.
StorageCondition	-20°C

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