Description | T4DNAPolymerasecatalyzestheextensionofaprimedDNAtemplate(inthepresenceoftemplateandprimer)inthe5′→3′direction.ThisD219AmutantT4DNAPolymerasedoesnotpossesseither3'->5’(proofreADIng)and5′→3′exonucleaseactivities. |
Applications | Randommutagenesis |
Source | ArecombinantE.colistraincarryingaclonedgene43(D219Amutant)ofbacteriophageT4. |
UnitDefinition | Oneunitisdefinedastheamountofenzymethatwillincorporate10nmolofdNTPintoacid-precipitablematerialin30minutesat37°C. |
Components |
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QualityControl | Theabsenceofendodeoxyribonucleasesconfirmedbyappropriatequalitytests. |
StorageCondition | -20°C |