Description | BsuDNAPolymeraseI,LargeFragmentcatalyzes5´→3´synthesisofDNAlacks5´→3´and3´→5´exonucleaseactivity. |
Applications | - Randomprimerlabeling
- SecondstrandCDNAsynthesis
- SingledAtailing
- StranddisplacementDNAsynthesis
- Recombinasepolymeraseamplification
|
Source | ArecombinantE.colistraincarryingthelargefragmentofBacillussubtilisDNApolymeraseIgene |
UnitDefinition | Oneunitisdefinedastheamountofenzymethatwillincorporate10nmolofdNTPintoacidinsolublematerialin30minutesat37°C. |
Components | - BsuDNAPolymerase:5,000U/mlin10mMTris-HCl,50mMKCl,1.0mMDithiothreitol,0.1mMEDTA,0.1%TritonX-100,50%Glycerol,pH7.5@25°C
- 10XReactionBuffer:500mMNaCl,100mMTris-HCl,100mMMgCl2,10mMDTTpH7.9@25°C
|
QualityControl | Theabsenceofendonuclease,nickingactivityandexonucleaseactivityisconfirmedbyappropriatequalitytests. |
StorageCondition | -20°C |
Excellgen蛋白表达产品名称-目录 #价钱293Expresso™转染试剂EG-1089$ 98.00定制抗体生产和免疫测定开发EG-36从培养基中纯化IgEEG-1076-1$ 750.00MembraneExpress TM功能膜蛋白的病毒样颗粒EG-1038蛋白质表达和纯化服务EG-1076-8$ 1,500.00小规模表达测试和纯化方法开发EG-1081-3$ 1,250.00使用HEK 293细胞的瞬时蛋白质生产EG-1080$ 2,900.00 $ 1,950.00-$ 950.00使用细菌表达系统进行蛋白表达和纯化EG-1076$ 2,170.00使用杆状病毒表达系统进行蛋白质表达和纯化EG-1078$ 4,500.00使用Akta FPLC系统的定制蛋白质纯化服务EG-1082$ 1,000.00使用稳定的哺乳动物细胞系表达蛋白质EG-1081$ 9,500.00