Description | RecombinantBstDNAPolymerase(largefragment)has5’->3’polymeraseactivity,butlacks5’->3’exonucleaseactivity.ThisenzymecanbeusedforandrapidamplificationoftraceamountsofDNAtemplatesforsequencing. |
Applications | - DNAsequencingthroughhighGCregions
- RapidamplificationoftraceamountsofDNAtemplates
- NextGenerationsequencing
|
Source | ArecombinantE.colistraincarryingthelargefragmentofDNApolymerasegenefromBacillusstearoThermophilus |
UnitDefinition | Oneunitisdefinedastheamountofpolymeraserequiredtocatalyzetheincorporationof10nmolofdNTPintoacid-insolublematerialin30minutesat65°C. |
Components | - BstDNAPolymerase:8,000U/mlin10mMTris-HCl,50mMKCl,1.0mMDithiothreitol,0.1mMEDTA,0.1%TritonX-100,50%Glycerol,pH7.5@25°C
- 10XReactionBuffer:200mMTris-HCl,100mM(NH4)2SO4,100mMKCl,20mMMgSO4,and1.0%TritonX-100,pH8.8@25°C
|
QualityControl | Theabsenceofendonuclease,nickingactivityandexonucleaseactivityisconfirmedbyappropriatequalitytests. |
StorageCondition | -20°C |
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