Description | Terminaldeoxynucleotidyltransferase(TdT)isatemplate-independentDNApolymerasethatcatalyzestheadditionofdeoxynucleotidestothe3'hydroxylterminusofsingleordoublestrandedDNAmolecules.Thepresenceof1mMCo2+stimulatesthetailingofthe3'-endsofDNAfragments. |
Applications | - Additionofhomopolymertailstothe3'endsofDNA
- Labelingthe3'endsofDNAwithmodifiednucleotides(e.g.,ddNTP,DIG-dUTP)
- TUNELassay(insitulocalizationofapoptosis)
- TdTdependentPCR
|
Source | ArecombinantE.colistraincarryinganengineeredhumanterminaldeoxynucleotidyltransferasegene |
UnitDefinition | 1unitisdefinedastheamountofpolymeraserequiredtoconvert1nmolofdATPsintoacidinsolublematerialin1hourat37°C. | |
Components | - TerminaldeoxynucleotidylTransferase:20,000U/mlin50mMKPO4,100mMNaCl,1.0mMDTT,0.1mMEDTA,50%Glycerol,0.1%TritonX-100,pH7.3@25°C
- 10XReactionBuffer:200mMTris-Acetate,500mMPotassiumAcetate,100mMMagnesiumAcetate,10mMDTT,pH7.9@25°C
- 2.5mMCoCl2
|
StorageCondition | -20°C |
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