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Excellgen/T7 DNA Polymerase/EG-1059/5,000 Units

价格
¥5000.00
货号:EG-1059-5,000Units
浏览量:127
品牌:Excellgen
服务
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商品描述
Description

T7DNAPolymerasecatalyzesDNAsynthesisinthe5'->3'direction.ItisahighlyprocessiveDNApolymeraseallowingcontinuoussynthesisoflongstretchesofDNA.Theenzymealsoexhibitsaverystrong3'->5'exonucleaseactivitytowardssingle-anddouble-strandedDNA.

Applications
  • PurificationofcovalentlyclosedcircularDNAbyremovalofresidualgenomicDNA
  • Primerextensionreactionsonlongtemplates
  • DNA3'-endlabeling
  • Strandextensionsinsite-directedmutagenesis
  • Fill-inbluntingof5'-overhangDNA
  • SecondstrandsynthesisofCDNA
  • InsitudetectionofDNAfragmentationassociatedwithapoptosis
SourceT7DNAPolymeraseiscomposedoftwosubunits:an80kDapolypeptide(theproductofgene5ofbacteriophageT7)anda12kDathioredoxinfromthetrxAgeneofE.coli.EachproteinisclonedandoverexpressedseparatelyinE.coli.
UnitDefinitionOneunitisdefinedastheamountofenzymethatwillincorporate10nmolofdNTPintoacid-precipitablematerialin30minutesat37°C.
Components
  • T7DNAPolymerase:10,000U/mlin50mMKPO4,0.1mMEDTA,1.0mMDTT,50%glycerol,pH7.0@25°C
  • 10XReactionBuffer:200mMTris-HCl,100mMMgCl210mMDTT,pH7.5@25°C
QualityControlTheabsenceofendodeoxyribonucleasesisconfirmedbytheappropriatequalitytest.
StorageCondition-20°C
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