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蚂蚁淘在线 / 品牌中心 / Excellgen / Excellgen/FastÄQ™ One-Step SYBR Green qRT-PCR Kit, ROX™/EG-1024/10 ml

Excellgen/FastÄQ™ One-Step SYBR Green qRT-PCR Kit, ROX™/EG-1024/10 ml

价格
￥30000.00
货号：EG-1024-10ml
浏览量：127
品牌：Excellgen
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商品描述
Description

FastÄQTMOne-StepSYBRGreenqRT-PCRKit,ROX™isaconvenientandhighlysensitivesolutionforquantitativeRT-PCRofRNAtemplates(qRT-PCR)usingSYBR®GreenIdyedetectionandgene-specificprimersonallAppliedBiosystemsreal-timePCRsystemsexceptthe7500.CDNAsynthesisandPCRamplificationarecarriedoutinthesametubewithoutopeningbetweenprocedures.ThesystemhasbeenoptimizedtodelivermaximumRTPCRefficiency,sensitivity,andspecificity.TheproprietaryreactionbufferhasbeenspecificallyformulatedtomaximizeactivitiesofbothreversetranscriptaseandTaqDNApolymerasewhileminimizingthepotentialforprimer-dimerandothernon-specificPCRartifacts.ThekitiscompatIBLewithbothfastandstandardqPCRcyclingprotocols.HighlyspecificamplificationisessentialforsuccessfulqRT-PCRwithSYBR®GreenItechnology,sincethisdyebindstoanydsDNAgeneratedduringamplification.FastÄQTMTaqDNApolymerasecontainsmonoclonalantibodiesthatbindtothepolymeraseandkeepitinactivepriortotheinitialPCRdenaturationstep.Uponheatactivationat95ºC,theantibodiesdenatureirreversibly,releasingfullyactive,unmodifiedTaqDNApolymerase.

InstrumentCompatibility

  • AppliedBiosystems5700
  • AppliedBiosystems7000
  • AppliedBiosystems7300
  • AppliedBiosystems7700
  • AppliedBiosystems7900
  • AppliedBiosystems7900HT
  • AppliedBiosystems7900HTFast
  • AppliedBiosystemsStepOne™
  • AppliedBiosystemsStepOnePlus™
Features
  • HighsensitivityFastÄQTaqDNAPolymerase
  • Hot-starttechnologyensureshighspecificity
Components
  • FastÄQTMOne-StepReverseTranscriptase-Optimized50XformulationofrecombinantMMLVreversetranscriptaseforone-stepRT-PCR.
  • One-StepSYBRGreenMasterMix,ROX(2X)-2XreactionbuffercontainingdNTPs,magnesiumchloride,FastÄQTaqDNApolymerase,stABIlizers,ROXreferencedyeandSYBRGreenIdye
  • Nuclease-freewater
StorageKitcomponentsarestableforoneyearwhenstoredinaconstanttemperaturefreezerat-20°C,protectedfromlight.Forconvenience,theOne-StepSYBRGreenMasterMix,ROXmaybestoredunfrozenat+2to+8°Cforupto4months.
Shipping-20°C
NoteForresearchuseonly
Excellgen蛋白表达产品名称-目录 ＃价钱293Expresso™转染试剂EG-1089$ 98.00定制抗体生产和免疫测定开发EG-36从培养基中纯化IgEEG-1076-1$ 750.00MembraneExpress TM功能膜蛋白的病毒样颗粒EG-1038蛋白质表达和纯化服务EG-1076-8$ 1,500.00小规模表达测试和纯化方法开发EG-1081-3$ 1,250.00使用HEK 293细胞的瞬时蛋白质生产EG-1080$ 2,900.00  $ 1,950.00-$ 950.00使用细菌表达系统进行蛋白表达和纯化EG-1076$ 2,170.00使用杆状病毒表达系统进行蛋白质表达和纯化EG-1078$ 4,500.00使用Akta FPLC系统的定制蛋白质纯化服务EG-1082$ 1,000.00使用稳定的哺乳动物细胞系表达蛋白质EG-1081$ 9,500.00