Description | 2XÄQStartTMTaqMastermixisformulatedforfasterandeasierpreparationofPCRreactions,requiringonlytheadditionofprimers,templatesandwater.AreductioninthenumberofpipettingstepsalsoensuresmorereproducIBLePCR. ÄQStartTMTaqDNApolymerasecontainspurifiedmonoclonalantibodiesthatbindtothepolymeraseandkeepitinactivepriortotheinitialPCRdenaturationstep.Uponheatactivation(1minuteat94°C),theantibodiesdenatureirreversibly,releasingfullyactive,unmodifiedTaqDNApolymerase.Thisenablesspecificandefficientprimerextensionwiththeconvenienceofroomtemperaturereactionassembly. |
Applications | RoutinePCRamplificationofupto4kbDNAfragment. |
Features |
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Components | Finalconcentration(1X):
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StorageConditions | -20°C |
StABIlity | 3yearsat-80°C;2yearsat-20°C,upto1yearat4°C.Repeatedfreeze/thawcyclesshouldbeavoidedbutdoesnotaffectproductperformancesignificantly. |
Shipping | 4°C |
QC | TopRow:PlatinumMix BottomRow:ÄQStartTMTaqMastermix Template:1:2serialdilutionpGEM3zf(+)startingconcentration12.5ng/µl |