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Excellgen/HiFi ÄQStartTM Taq Mastermix/EG-1017/500 Reactions

价格
¥13360.00
货号:EG-1017-500Reactions
浏览量:127
品牌:Excellgen
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商品描述
Description

2XHiFiÄQStartTMTaqMastermixisformulatedforfasterandeasierpreparationofPCRreactions,requiringonlytheadditionofprimers,templatesandwater.AreductioninthenumberofpipettingstepsalsoensuresmorereproducIBLePCR.

HiFiÄQStartTMTaqDNApolymerasecontainspurifiedmonoclonalantibodiesthatbindtothepolymeraseandkeepitinactivepriortotheinitialPCRdenaturationstep.Uponheatactivation(1minuteat94°C),theantibodiesdenatureirreversibly,releasingfullyactive,unmodifiedTaqDNApolymerase.Thisenablesspecificandefficientprimerextensionwiththeconvenienceofroomtemperaturereactionassembly.

HiFiÄQStartTMTaqDNApolymerasecontainsasmallamountofultrahighfidelitythermalstableDNApolymerasewith3’→5’exonucleaseactivity.Thisleadstoupto6-foldhigherfidelityPCRamplificationthanTaqDNApolymerasealoneandenablesamplificationofDNAfragmentsupto20-kb.

Applications
  • RoutinePCRamplificationofupto20kbDNAfragmentfollowedbycloningofPCRproduct
  • LongPCR.
Features
  • Highfidelity,highyield,highsensitivityÄQStartTaqDNAPolymerase
  • Convenientpre-mixed,pre-optimized2xsolutions
  • Hot-starttechnologyensuresspecificandefficientprimerextension
  • Superiorreproducibilityandprecision
  • Convenientreactionassemblyatroomtemperature
  • Dramaticallydecreasesthetimerequiredforreactionset-up
  • Avoidstiresomemanipulationssuchasthawingofenzyme,dilutionofenzyme,pipettingofdNTPsandMgCl2.
  • Stableat4°C
ComponentsFinalconcentration(1X):
  • RecombinantÄQStartTaqDNAPolymerase:0.02U/µl
  • dNTPs,each:100µM
  • Tris-HClpH9.0:10mM
  • KCl:50mM
  • MgCl2:1.5mM
  • TritonX-100:0.1%
  • BSA:0.2mg/ml
  • Glycerol:1.8%
  • ProprietaryPCRenhancer
StorageConditions-20°C
StABIlity3yearsat-80°C;2yearsat-20°C,upto1yearat4°C.Repeatedfreeze/thawcyclesshouldbeavoidedbutdoesnotaffectproductperformancesignificantly.
Shipping4°C
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