Description | HiFiÄQStartTMTaqDNApolymeraseisanenzymemixtureofrecombinantTaqDNApolymerasepreparation,athermalstableDNApolymerasewith3’→5’exonucleaseactivity,andmonoclonalantibodiesthatbindtothepolymeraseandkeepitinactivepriortoPCRthermalcycling.Uponheatactivation(1minuteat94°C),theantibodiesdenatureirreversIBLy,releasingfullyactive,unmodifiedTaqDNApolymerase.Thisenablesspecificandefficientprimerextensionwiththeconvenienceofroomtemperaturereactionassembly.ThisenzymemixtureandoptimizedHiFiPCRbufferenablesupto6foldhigherfidelitythanTaqDNApolymeraseandallowsamplificationofDNAfragmentsupto20-kb. |
Applications | RoutinePCRamplificationofupto20kbDNAfragmentfollowedbycloningoftheamplifiedfragment. |
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Storage | HiFiÄQStartTMTaqDNApolymeraseisstablefor2yearswhenstoredinaconstanttemperaturefreezerat-20°C. |
Shipping | 4°C |