ProductName	Farnesoid-X-activatedReceptor,FarnesoidXreceptor
Size	10,000U
Description	Farnesoid-X-receptor(FXR)wasoriginallyidentifiedandclonedinratasanorphannuclearhormonereceptorbasedonhybridizationwithadegenerateoligonucleotidedesignedfromthehighlyconservednuclearhormonereceptorDNAbindingdomain(1).FXRfunctionsasaheterodimerwithRXRandbindstosequenceelementsinthepromotersoftargetgenes.TheFXR/RXRheterodimerbindswithhighestaffinitytoinvertedrepeatsseparatedby1bp(IR-1)andwithlowaffinitytodirectrepeatsseparatedby4and5bp(DR-4andDR-5)(1,2).Asisthecaseforothernuclearhormonereceptors,FXRregulatestargetgeneactivityinresponsetoligand.WhileinitialstudiessuggestedthatfarnesolandretinoidmetaboliteswerelikelyligandsforFXR,currentdatasupportthenotionthatFXRisabileacidsensorthatplaysanintegralroleinbileacidsynthesisandtransport(1,3-6).Inthesmallintestine,FXRregulatesbileaciduptakethroughtheupregulationoftheilealbileacidbindingproteingeneviabindingtoanupstreamresponseelement(7).TheFXR/RXRheterodimercanbeactivatedbythebilesaltchenodeoxycholicacid(CDCA)andFXRisrequiredforthebilesalt-dependenttranscriptionalcontrolofthehumanABCB11gene(thebilesaltexportpump)(8).Inaddition,FXRhasbeenshowntoinhibitthecholesterol7-hydrolasegene(CYP7A1)transcription(9).
Applications	FXRhasbeenappliedinDNAandprotein-proteininteractionsassays
Source	E.coli
FusionTag	HisTag
PurificationMethod	FPLC
UnitDefinition(Activity)	1unitequalsto1nanogrampurifiedprotein.20unitsaresufficientforagel-mobilityshiftassayand100unitsaresufficientforaprotein-proteininteractionassay
Purity	~95%asdeterminedbySDS-PAGE
GeneID	9971
GeneName	FXR
GeneSynonyms	BAR;FXR;HRR1;HRR-1;RIP14
MW	56.6kDa
ProteinSequence	MGSKMNLIEHSHLPTTDEFSFSENLFGVLTEQVAGPLGQNLEVEPYSQYSNVQFPQVQPQ ISSSSYYSNLGFYPQQPEEWYSPGIYELRRMPAETLYQGETEVAEMPVTKKPRMGASAGR IKGDELCVVCGDRASGYHYNALTCEGCKGFFRRSITKNAVYKCKNGGNCVMDMYMRRKCQ ECRLRKCKEMGMLAECLLTEIQCKSKRLRKNVKQHADQTVNEDSEGRDLRQVTSTTKSCR EKTELTPDQQTLLHFIMDSYNKQRMPQEITNKILKEEFSAEENFLILTEMATNHVQVLVE FTKKLPGFQTLDHEDQIALLKGSAVEAMFLRSAEIFNKKLPSGHSDLLEERIRNSGISDE YITPMFSFYKSIGELKMTQEEYALLTAIVILSPDRQYIKDREAVEKLQEPLLDVLQKLCK IHQPENPQHFACLLGRLTELRTFNHHHAEMLMSWRVNDHKFTPLLCEIWDVQ
StorageBuffer	20mMTris-HCl,pH8.0,250mMNaCl,50%Glycerol
Storage	-20to-80°C.
Shipping	4°Cordryice
References	1.Formanetal.,(1995)Cell81,687-693 2.Seoletal.,(1995)Mol.Endocrinol.9,72-85 3.Zavackietal.,(1997)Proc.Natl.Acad.Sci.94,7909-7914 4.Makishimaetal.,(1999)Science284,1362-1365 5.Parksetal.,(1999)Science284,1365-1368 6.Wangetal.,(1999)Mol.Cell3,543-553 7.Groberetal.,(1999)J.Biol.Chem.274,29749-29754 8.Plassetal.,(2002)Hepatology35,589-596 9.Chiangetal.,(2001)Gene262,257-265

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