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商品描述
| ProductName | Farnesoid-X-activatedReceptor,FarnesoidXreceptor |
| Size | 10,000U |
| Description | Farnesoid-X-receptor(FXR)wasoriginallyidentifiedandclonedinratasanorphannuclearhormonereceptorbasedonhybridizationwithadegenerateoligonucleotidedesignedfromthehighlyconservednuclearhormonereceptorDNAbindingdomain(1).FXRfunctionsasaheterodimerwithRXRandbindstosequenceelementsinthepromotersoftargetgenes.TheFXR/RXRheterodimerbindswithhighestaffinitytoinvertedrepeatsseparatedby1bp(IR-1)andwithlowaffinitytodirectrepeatsseparatedby4and5bp(DR-4andDR-5)(1,2).Asisthecaseforothernuclearhormonereceptors,FXRregulatestargetgeneactivityinresponsetoligand.WhileinitialstudiessuggestedthatfarnesolandretinoidmetaboliteswerelikelyligandsforFXR,currentdatasupportthenotionthatFXRisabileacidsensorthatplaysanintegralroleinbileacidsynthesisandtransport(1,3-6).Inthesmallintestine,FXRregulatesbileaciduptakethroughtheupregulationoftheilealbileacidbindingproteingeneviabindingtoanupstreamresponseelement(7).TheFXR/RXRheterodimercanbeactivatedbythebilesaltchenodeoxycholicacid(CDCA)andFXRisrequiredforthebilesalt-dependenttranscriptionalcontrolofthehumanABCB11gene(thebilesaltexportpump)(8).Inaddition,FXRhasbeenshowntoinhibitthecholesterol7-hydrolasegene(CYP7A1)transcription(9). |
| Applications | FXRhasbeenappliedinDNAandprotein-proteininteractionsassays |
| Source | E.coli |
| FusionTag | HisTag |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogrampurifiedprotein.20unitsaresufficientforagel-mobilityshiftassayand100unitsaresufficientforaprotein-proteininteractionassay |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 9971 |
| GeneName | FXR |
| GeneSynonyms | BAR;FXR;HRR1;HRR-1;RIP14 |
| MW | 56.6kDa |
| ProteinSequence | MGSKMNLIEHSHLPTTDEFSFSENLFGVLTEQVAGPLGQNLEVEPYSQYSNVQFPQVQPQ ISSSSYYSNLGFYPQQPEEWYSPGIYELRRMPAETLYQGETEVAEMPVTKKPRMGASAGR IKGDELCVVCGDRASGYHYNALTCEGCKGFFRRSITKNAVYKCKNGGNCVMDMYMRRKCQ ECRLRKCKEMGMLAECLLTEIQCKSKRLRKNVKQHADQTVNEDSEGRDLRQVTSTTKSCR EKTELTPDQQTLLHFIMDSYNKQRMPQEITNKILKEEFSAEENFLILTEMATNHVQVLVE FTKKLPGFQTLDHEDQIALLKGSAVEAMFLRSAEIFNKKLPSGHSDLLEERIRNSGISDE YITPMFSFYKSIGELKMTQEEYALLTAIVILSPDRQYIKDREAVEKLQEPLLDVLQKLCK IHQPENPQHFACLLGRLTELRTFNHHHAEMLMSWRVNDHKFTPLLCEIWDVQ |
| StorageBuffer | 20mMTris-HCl,pH8.0,250mMNaCl,50%Glycerol |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Formanetal.,(1995)Cell81,687-693 |
