ProductName	RetinoidXReceptor-alphaLigandBindingDomain
Size	10,000U
Description	RetinoidXreceptor(RXR)servesasapromiscuousheterodimerizationpartnerformanynuclearreceptorsthroughtheidentitybox,a40-aminoacidsubregionwithintheligandbindingdomain(LBD).RXRpartnersincludethyroidhormonereceptors(TRs),retinoicacidreceptors(RARs),peroxisomeproliferator-activatedreceptor,severalconstitutiveactiveorphannuclearreceptors(e.g.nucleargrowthfactorI-B),oxysterolreceptors,andconstitutiveandrostanereceptors.RXRsalsoformhomodimerstomediatetheeffectsof9-cis-retinoicacid(9-cRA).Dependingontheseprotein-proteininteractions,RXR-containingcomplexeshavedistinctligand-dependentandconstitutivefunctions(1,2).TheLBDisfunctionallycomplexandmediatesligandbinding,receptorhomo-andheterodimerization,repressionoftranscriptionintheabsenceofligand,andligand-dependentactivationoftranscription(3).HormonebindingtothestructurallyconservedLBDoftheRXRtriggersaconformationalchangethatprincipallyaffectstheconservedC-terminaltransactivationhelixH12involvedintranscriptionalactivation(4).Coactivatorsdirectlyrecruitedbyligandedreceptorsincludemembersofthesteroidreceptorcoactivator/p160familysuchasSRC-1,transcriptionalintermediaryfactor2/glucocorticoidreceptorinteractingprotein1,andRAC3/activatorofthyroidandretinoicacidreceptors/amplifiedinbreastcancer1(5).
Applications	invitrotranscriptionassays DNAandprotein-proteininteractionsassays
Source	E.coli
FusionTag	6XHis
PurificationMethod	FPLC
UnitDefinition(Activity)	1unitequalsto1nanogrampurifiedprotein.20unitsaresufficientforaninvitrotranscriptionassayand100unitsaresufficientforaligandbindingoraprotein-proteininteractionassay
Purity	~95%asdeterminedbySDS-PAGE
GeneID	6256
Accession#	NM_002957
GeneName	retinoidXreceptor,alpha
GeneSymbol	RXRA
GeneSynonyms	NR2B1
MW	32.0kDa
ProteinSequence	MGMKREAVQEERQRGKDRNENEVESTSSANEDMPVERILEAELAVEPKTETYVEANMGLN PSSPNDPVTNICQAADKQLFTLVEWAKRIPHFSELPLDDQVILLRAGWNELLIASFSHRS IAVKDGILLATGLHVHRNSAHSAGVGAIFDRVLTELVSKMRDMQMDKTELGCLRAIVLFN PDSKGLSNPAEVEALREKVYASLEAYCKHKYPEQPGRFAKLLLRLPALRSIGLKCLEHLF FFKLIGDTPIDTFLMEMLEAPHQMT
StorageBuffer	20mMTris-HCl,pH8.0,250mMNaCl,50%Glycerol
Storage	-20to-80°C.
Shipping	4°Cordryice
References	1.Mangelsdorfetal.,(1995)Cell83,841-850 2.Mangelsdorfetal.,(1995)Cell83,835-839 3.Nakashimaetal.,(1999)Science284,479-482 4.Egeaetal.,(2001)J.Mol.Biol.307,557-576 5.Leoetal.,(2000)Gene245,1-11

Excellgen蛋白表达产品名称-目录 ＃价钱293Expresso™转染试剂EG-1089$ 98.00定制抗体生产和免疫测定开发EG-36从培养基中纯化IgEEG-1076-1$ 750.00MembraneExpress TM功能膜蛋白的病毒样颗粒EG-1038蛋白质表达和纯化服务EG-1076-8$ 1,500.00小规模表达测试和纯化方法开发EG-1081-3$ 1,250.00使用HEK 293细胞的瞬时蛋白质生产EG-1080$ 2,900.00  $ 1,950.00-$ 950.00使用细菌表达系统进行蛋白表达和纯化EG-1076$ 2,170.00使用杆状病毒表达系统进行蛋白质表达和纯化EG-1078$ 4,500.00使用Akta FPLC系统的定制蛋白质纯化服务EG-1082$ 1,000.00使用稳定的哺乳动物细胞系表达蛋白质EG-1081$ 9,500.00