ProductName	Nuclearreceptorcoactivator1,SRC1-RIDBiotinylated
Size	5,000U
Description	Steroidreceptorcoactivator1(SRC1)isatranscriptionalcoactivoatorthatmediatestheactivatingfunctionsofmanyofthenuclearhormonereceptors.ItisalsoknownasNCoA1andisamemberoftheSRC/p160coactivatorfamily(1).SRC1hasbeenshowntobeoverexpressedinsomecancers(2).SRC1isa160kDaproteinthatcontainsseveralLXXLLmotifs,whichareinvolvedinnuclearreceptorinteraction(3).Theregion627-786contains3LXXLLmotifsthatareinvolvedininteractionwithnuclearhormonereceptors(4,5)andhasbeenpreviouslyusedinassaysdetectingligand-dependentreceptor-cofactorinteractions(6).ThisproteindoesnotcontainHistagandwillbeusefulforassayswhereanativeuntaggedproteinisdesired.Lane1correspondsto6His-taggedandbiotinylatedSRC-RID;Lane2correspondstobiotinylatedSRC-RIDwithout6Histag.
Applications	protein-proteininteractionassays,particularlysuitableforhighthroughputscreening(HTS)assays.
Source	E.coli
FusionTag	None
PurificationMethod	FPLC
UnitDefinition(Activity)	1unitequalsto1nanogramofpurifiedprotein.100unitsaresufficientforaprotein-proteininteractionassay.
Purity	~95%asdeterminedbySDS-PAGE
GeneID	8648
Accession#	NM_003743
GeneName	NCOA1
GeneSynonyms	SRC1;KAT13A;RIP160;F-SRC-1;bHLHe42;bHLHe74
MW	19.8kDa
ProteinSequence	SQTSHKLVQLLTTTAEQQLRHADIDTSCKDVLSCTGTSNSASANSSGGSCPSSHSSLTER HKILHRLLQEGSPSDITTLSVEPDKKDSASTSVSVTGQVQGNSSIKLELDASKKKESKDH QLLRYLLDKDEKDLRSTPNLSLDDVKVKVEKKEQMDPCNT
StorageBuffer	20mMTris-HCl,pH8.0,250mMNaCl,50%Glycerol
Storage	-20to-80°C.
Shipping	4°Cordryice
References	1.Onate,S.etal.,(1995)Science270,1354-1357 2.Yanase,T.,etal.,(2004)Intern.Med.43,368-373 3.Heery,D.etal.,(1997)Nature387,733-736 4.Xu,J.andLi,Q.,(2003)Mol.Endocrinol.17,1681-1692 5.Bai,Y.andGiguere,V.,(2003)Mol.Endocrinol.17,589-599 6.Cho,M.etal.,(2003)J.Biochem.Mol.Biol.36,207-213

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