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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
| Description | ThermostableATPindependentRNALigase2specificallyligatesthepreadenylated5´endofssDNAorRNAtothe3´OHendofRNAat60–65°C.TheenzymedoesnotrequireATPforligationbutrequirespre-adenylatedlinkers. |
| Applications | Ligatea5´pre-adenylated3´-blockedDNAorRNAsequencetagtothe3´-OHofRNAandssDNA. |
| Source | PurifiedfromarecombinantE.colistraincarryingathermostablearchaealRNALigase |
| Components |
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| Activity | 1µgproteinwillligateatleast16pmolofpre-adenylateddonorsubstrateRNAto10pmolofacceptorsubstrate(e.g.17merssDNA)inatotalreactionvolumeof200μlin1hourat65°C. |
| QualityControl | Theabsenceofendo-,exodeoxyribonucleases,phosphataseandribonucleasesconfirmedbyappropriatequalitytests. |
| Storage | -20°C. |


