Description | ThermostableATPindependentRNALigase2specificallyligatesthepreadenylated5´endofssDNAorRNAtothe3´OHendofRNAat60–65°C.TheenzymedoesnotrequireATPforligationbutrequirespre-adenylatedlinkers. |
Applications | Ligatea5´pre-adenylated3´-blockedDNAorRNAsequencetagtothe3´-OHofRNAandssDNA. |
Source | PurifiedfromarecombinantE.colistraincarryingathermostablearchaealRNALigase |
Components |
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Activity | 1µgproteinwillligateatleast16pmolofpre-adenylateddonorsubstrateRNAto10pmolofacceptorsubstrate(e.g.17merssDNA)inatotalreactionvolumeof200μlin1hourat65°C. |
QualityControl | Theabsenceofendo-,exodeoxyribonucleases,phosphataseandribonucleasesconfirmedbyappropriatequalitytests. |
Storage | -20°C. |