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Excellgen/T4 RNA Ligase 2, ATP independent/EG-146/2,000 Units

价格
¥1000.00
货号:EG-146-2,000Units
浏览量:127
品牌:Excellgen
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商品描述
Description

ATPindependentT4RNALigase2specificallyligatesthepreadenylated5´endofDNAorRNAtothe3´OHendofRNA.TheenzymedoesnotrequireATPforligationbutrequirespre-adenylatedlinkers.

Applications
  • Ligateapre-adenylatedDNAorRNAsequencetagtoanyRNA3´-end
  • JoinasinglestrandedadenylatedprimertosmallRNAsforCDNAlibrarycreation
  • JoinasinglestrandedadenylatedprimertoRNAforstrand-specificcDNAlibraryconstruction.
SourcePurifiedfromarecombinantE.colistraincarryingamutantofT4RNALigase2.
Components
  • ATPindependentT4RNALigase2,200,000​units/mlinstoragebuffer:10mlTris-HCl,100mMNaCl,0.1mMEDTA,0.1mMDTT,50%Glycerol,pH7.5@25°C
  • 10XT4RNALigase2truncatedBuffer:500mMTris-HCl,100mMMgCl2,10mMDTT,pH7.5@25°C
UnitDefinition

200unitsisdefinedastheamountofenzymerequiredtogive80%ligationofa31-merRNAtothepre-adenylatedendofa17-merDNAinatotalreactionvolumeof20μlin1hourat25°C.

5´-FAM-rArGrUrCrGrUrArGrCrCrUrUrUrArUrCrCrGrArGrArUrUrCrArGrCrArArUrA-3´
5´-rAppCTGTAGGCACCATCAAT–NH2-3´

QualityControl

Theabsenceofendo-,exodeoxyribonucleases,phosphataseandribonucleasesconfirmedbyappropriatequalitytests.

Storage-20°C.
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