品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Description | T4RNALigase2truncatedcatalyzesphosphodiesterbondformationbetweenapre-adenylated5′phosphate(DNAorRNA)andthe3′hydroxylofRNA.Thetruncatedenzymecontainingthefirst249aminoacidsisunabletoadenylatethe5´endofthesubstrate,thusitcannotligatethephosphorylated5´endofRNAorDNAtothe3´endofRNA.Suchapropertyisusefulforcertainapplicationssuchaslinkerligationswithpre-adenylated5′DNAto3′hydroxylRNA,suchasmicroRNAs.Thistruncatedenzymesignificantlyreducesunwantedbackgroundligationasitcanonlyuseadenylatedprimers. |
Applications |
|
Source | PurifiedfromarecombinantE.colistraincarryingtheT4phageRNALigase2Rnl2(gp24.1)(1-249)gene |
Components |
|
UnitDefinition | Oneunitisdefinedastheamountofenzymerequiredtoligate50%of0.4µgofanequimolarmixofasingle-stranded5′FAM-labeled17-merRNAtothe5′pre-adenylatedendofa18-merDNAwhenboth17-mersareannealedtoacomplementary35-merDNAstrandin20μL1Xreactionbufferfollowinga30minuteincubationat37°C. |
QualityControl |
|
Storage | -20°C. |