Description	T4RNALigase2truncatedcatalyzesphosphodiesterbondformationbetweenapre-adenylated5′phosphate(DNAorRNA)andthe3′hydroxylofRNA.Thetruncatedenzymecontainingthefirst249aminoacidsisunabletoadenylatethe5´endofthesubstrate,thusitcannotligatethephosphorylated5´endofRNAorDNAtothe3´endofRNA.Suchapropertyisusefulforcertainapplicationssuchaslinkerligationswithpre-adenylated5′DNAto3′hydroxylRNA,suchasmicroRNAs.Thistruncatedenzymesignificantlyreducesunwantedbackgroundligationasitcanonlyuseadenylatedprimers.
Applications	Ligateapre-adenylatedDNAorRNAsequencetagtoanyRNA3´-end JoinasinglestrandedadenylatedprimertosmallRNAsforCDNAlibrarycreation JoinasinglestrandedadenylatedprimertoRNAforstrand-specificcDNAlibraryconstruction
Source	PurifiedfromarecombinantE.colistraincarryingtheT4phageRNALigase2Rnl2(gp24.1)(1-249)gene
Components	T4RNALigase2truncated:200,000units/mlinstoragebuffer:10mMTris-HCl,100mMNaCl,0.1mMDTT,0.1mMEDTA,50%glycerol,pH7.5@25°C 10XT4RNALigase2truncatedBuffer:500mMTris-HCl,100mMMgCl2,50mMDTT,pH7.6@25°C
UnitDefinition	Oneunitisdefinedastheamountofenzymerequiredtoligate50%of0.4µgofanequimolarmixofasingle-stranded5′FAM-labeled17-merRNAtothe5′pre-adenylatedendofa18-merDNAwhenboth17-mersareannealedtoacomplementary35-merDNAstrandin20μL1Xreactionbufferfollowinga30minuteincubationat37°C.
QualityControl	Theabsenceofendo-,exodeoxyribonucleases,phosphatases,andribonucleasesconfirmedbyappropriatequalitytests. Functionallytestedforthecapacitytojoincohesive-andblunt-endDNAfragments.
Storage	-20°C.

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