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蚂蚁淘在线 / 品牌中心 / Excellgen / Excellgen/Chlorella Virus PBCV-1 DNA Ligase/EG-1063/5,000 Units

Excellgen/Chlorella Virus PBCV-1 DNA Ligase/EG-1063/5,000 Units

价格
￥7960.00
货号：EG-1063-5,000Units
浏览量：127
品牌：Excellgen
服务
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商品描述
Description

ChlorellavirusPBCV-1DNAligase(ChlorellavirusDNAligase,alsoknownasSplintR™LigasesoldbyNEB),isanATP-dependentDNAligase.PBCV-1DNAligaseefficientlycatalyzestheligationofadjacent,single-strandedDNAsplintedbyacomplementaryRNAstrand.ThisunusualactivitycanbeusedtocharacterizemiRNAs,RNAsandSNPs,e.g.sensitive(sub-nanomolarrange)detectionofspecificRNAbyligation.Inaddition,PBCV-1DNAligasemaybeusedforenrichmentofRNAfornext-generationsequencing.

PBCV-1DNAligaseenzymeisactiveoverabroadrangeofATPconcentrations(10µM–1mM)andpH(6.5–9).OptimalactivityisobservedusingMg2+>5mM,andapHbetween7.5and8.0.Theactivityisenhancedatelevatedtemperatures(upto37°C),andbysupplementationwith5mMMn2+.Thereactionisinhibitedbysaltconcentrationswhenconcentrationis>100mM.

Theenzymetoleratesallbasepaircombinationsattheligationjunction,butispartiallyinhibitedbydC/GanddG/Cbasepairsatthedonor(phosphorylated)sideligationjunction,particularlywhenthe+2basewasalsoaC/Gbasepair.

Applications
  • LigationofssDNAsplintedbycomplementaryRNAsequences
  • DetectionofRNAusingligationofDNAprobes
  • SNPorsplicevariantdetection
  • RASL-seq
SourcePurifiedfromarecombinantE.colistraincarryingthePBCV-1DNAligasegene
Components
  • ChlorellavirusPBCV-1DNAligase,10,000unitsinstoragebuffer:10mMTris-HCl,300mMNaCl,1mMDTT,0.1mMEDTA,50%Glycerol,pH7.4@25°C
  • 10XLigationBuffer:250mMTris-HCI,50mMMgCl2,5mMDTT,10mMATP,pH7.6@25°C
UnitDefinitionOneunitisdefinedastheamountofenzymeneededtoligate(to50%completion)2picomolesofatripartiteFAM-labeledDNA:RNAhybridsubstrateina20μlreactionat25°Cin15minutesin1XLigationBuffer.
QualityControl

Theabsenceofendo-,exodeoxyribonucleases,andribonucleasesconfirmedbyappropriatequalitytests.

Storage-20°C.
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