Description	T4DNALigasecatalyzestheformationofaphosphodiesterbondbetweentheterminal5′phosphateanda3′hydroxylgroupsofduplexDNAorRNA.TheenzymeefficientlyjoinsbluntandcohesiveendsandrepairssinglestrandednicksinduplexDNA,RNAorDNA/RNAhybrids.CohesiveligationwithT4DNALigasecanbecompletedinlessthan10min.
Applications	Cloningofrestrictionfragments Joininglinkersandadapterstoblunt-endedDNA Libraryconstruction TAcloning RecirculizationoflinearDNA
Source	PurifiedfromarecombinantE.colistraincarryingtheT4DNALigasegene
Components	T4DNALigase,600,000U/mlinstoragebuffer:10mMTris-HCl,50mMKCl,1mMDTT,0.1mMEDTA,50%glycerol,pH7.5@25°C. 4XT4DNALigationBuffer:250mMTris-HCI,40mMMgCl2,4mMDTT,4mMATP,30%PEG6000,pH7.6@25°C
UnitDefinition	1unitisdefinedastheamountofT4DNALigaserequiredtojoin50%of100ngofDNAfragmentswithcohesiveterminiin50µl1XT4DNALigaseBufferfollowinga30minuteincubationat23°C.
QualityControl	Theabsenceofendo-,exodeoxyribonucleases,phosphatases,andribonucleasesconfirmedbyappropriatequalitytests. Functionallytestedforthecapacitytojoincohesive-andblunt-endDNAfragments.
Storage	-20°C.

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