Description	EcoliRNA5´Pyrophosphohydrolase(RppH,alsoknownasNudH/YgdP)removespyrophosphatefromthe5´endoftriphosphorylatedRNAtoleavea5´monophosphateRNA.ThisenzymecansplitAp5AtoADPandATP.
Applications	RNAstABIlitystudies Removingaguaninecapfromthe5′endofanRNA
Source	PurifiedfromarecombinantE.colistraincarryingtheEcoliRppHgene
Components	EcoliRNA5´Pyrophosphohydrolase,5,000U/mlinstoragebuffer:20mMTris-HCl,200mMNaCl,1mMDTT,0.1mMEDTA,50%Glycerol,0.01%Triton®X-100,pH7.5@25°C 10XReactionBuffer:500mMNaCl,100mMTris-HCl,100mMMgCl2,10mMDTT,pH7.9@25°C
UnitDefinition	Oneunitistheamountofenzymethatconverts1µg300merRNAtranscriptintoaXRN-1digestIBLeRNAin30minutesat37°C.
QualityControl	Theabsenceofendonuclease,exonuclease,phosphatases,andRNaseactivitiesisconfirmedbyappropriatequalitytests.
Storage	-20°C.

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