Description | InorganicPyrophosphatasecatalyzesthehydrolysisofinorganicpyrophosphateintotwoorthophosphates.TheenzymerequiresadivalentmetalcationwithMg2+foractivity |
Applications | - HighyieldRNAsynthesisbyinvitrotranscription
- DNApolymerizationreactions:preventingaccumulationofpyrophosphate
- RemovalofcontaminantPPiinreagentsusedforSNPgenotypingbymethodsbasedonthedetectionofpyrophosphate
|
Source | PurifiedfromarecombinantE.colistraincarryingtheEcoliinorganicpyrophosphatase(ppa)gene |
Components | - InorganicPyrophosphatase,100U/mlinstoragebuffer:20mMTris-HCl,100mMNaCl,1mMDTT,0.1mMEDTA,50%Glycerol,pH8.0@25°C
- 10XReactionBuffer:200mMTris-HCl,20mMMgCl2,pH7.2@25°C
|
UnitDefinition | Oneunitistheamountofenzymethatwillgenerate1µmolofphosphateperminutefrominorganicpyrophosphateunderstandardreactionconditions(a10minutereactionat25°Cin20mMTris-HCl,pH7.2,2mMMgCl2and2mMPPi). |
QualityControl | Theabsenceofendo-,exodeoxyribonucleases,phosphatases,andribonucleasesisconfirmedbyappropriatequalitytests. |
Storage | -20°C. |
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