Description | CreRecombinaseexosome-likevesiclesare20to50nmlipidvesiclesisolatedfromculturedmammaliancells.ThesevesiclesencapsulatehighconcentrationofNLS-Crerecombinase,butdonotcontainvirusandnucleicacids(plasmidDNA,RNAetc).Additionof10~50µlofthevesiclestoreportercells(suchasHEK293,Hela,NIH3T3)andprimarycellsresultsinloxPrecombination.Thetransductionefficiencyrangesfrom60to99%. |
Applications |
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Size | 2000units |
Activity | Astandardof100UnitsisdefinedastheamountofHTN-Crein1.0mLoftissueculturemediumthatisrequiredtoinduce90%RFPexpressioninaHEK293Tloxpreportercelllineassay |
Endotoxin | <0.1EU/ug |
Storage | 4ºC~-80ºC |
Shipping | dryice |