Description | RecombinantCrerecombinase(TAT-Cre)waspurifiedfromanHEK293celllineexpressingenhancedformofCreRecombinasefrombacteriophageP1.ThisCrerecombinasehasanN-terminal6XHistag,aTatpeptide(GRKKRRQRRRPPAGTSVSL)andanNLSsequence(PKKKRKV).Tat-Creisthemosteffectiveproteinintransduction(invivo)andsubsequentrecombinationcomparedtootherformsofCrerecombinases,e.g.,HNC,TCH6,HC,HNCM,CH. |
Applications |
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Source | HEK293cells |
Activity | Astandardof100UnitsisdefinedastheamountofTat-Crein1.0mLoftissueculturemediumthatisrequiredtoinduce90%GFPexpressioninaHEK293TloxPreportercelllineassay. |
Properties | QC:HEK293T-Crereportercellline80~100%recombinationefficiencies. Figure1.QCassayofTat-Cre.Adding 50and100unitsofTat-CretoHEK293TcellstransfectedwithLoxP-RFP-Stop-LoxP-GFPreporterplasmidDNA. EndotoxinLevels:<0.1EU/ug |
Storage | -20ºC~-80ºC |
Shipping | dryice |