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蚂蚁淘在线 / 品牌中心 / Excellgen / Excellgen/Vaccinia Virus Topoisomerase I/EG-167/500 Units

Excellgen/Vaccinia Virus Topoisomerase I/EG-167/500 Units

价格
￥1960.00
货号：EG-167
浏览量：127
品牌：Excellgen
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商品描述
Description

VacciniavirusTopoisomeraseIisatypeIeukaryotictopoisomerasethatremovesbothpositiveandnegativesuperhelicalturns(alsocalledright-andleft-handedsupercoils)fromcovalentlyclosedDNA.Theproductofthereactionisacovalentlyclosed,circularDNAwithfewerpositiveornegativesuperhelicalturns.DNATopoisomeraseIdoesnotabsolutelyrequireMg2+tofunction,althoughlowconcentrationsofMg2+mayincreaseactivity

Applications

Studyingtheeffectsofsupercoilingontranscriptioninvitro.
Studyingchromatinreconstitutioninvitro.
DeterminingthedegreeofsupercoilingofnaturallyoccurringDNA.
Detectingmutantplasmidsthatdifferinlengthbyonlyonebasepair.
IncreasingrestrictionendonucleasedigestionofresistantDNAsubstratesbyunwindingtheDNAcoilstoexposerestrictionsites.

SourceAnE.colistrainthatcarriestheVacciniavirusH6Rgene

UnitDefinition

OneunitofVacciniavirusDNATopoisomeraseIwillconvert1µgofsupercoiledDNA(FormI)torelaxedclosedcircularDNA(FormII)in1hourat37°Cunderstandardassayconditions.
Components

VacciniavirusTopoisomeraseI:10,000units/mlin10mMTris-HCl,1mMDTT,0.1mMEDTA,50%glycerol,pH7.5@25°C

QualityControl

Theabsenceofexo-andendodeoxyribonucleases,non-specificDNaseandRNaseisconfirmedbyappropriatequalitytest.

StorageCondition

-20°C

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