Description | T4EndonucleaseV (T4pyrimidinedimerglycosylase, T4PDG),hasbothDNAN-glycosylaseandapurinic/apyrimidiniclyase(APlyase)activities.Thisenzyme recognizes cis-syn-cyclobutanepyrimidinedimersinDNAcausedbyultraviolet(UV)irrADIation,andtheendonucleolyticactivitycleavesthephosphodiesterbondattheAPsite. |
Applications |
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Source | PurifiedfromanE.colistraincarryingaplasmidencodingT4denVgene. |
Components | T4EndonucleaseV:10,000units/mlin10mMTris-HCl,100mMNaCl,0.1mMDTT,0.1mMEDTA,50%Glycerol,pH7.5@25°C |
UnitDefinition | OneunitofT4EndonucleaseVconverts1µgofUV-irradiatedplasmidDNAfromcovalentlyclosedcirclestonickedformin30minutesat37°C. |
QualityControl | Theabsenceofnonspecificexo-andendonucleaseactivitiesandRNaseactivityisconfirmedbyappropriatequalitytest. |
StorageCondition | -20°C |