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Excellgen/T4 Endonuclease V, T4 PDG/EG-161/10,000 Units

价格
¥5360.00
货号:EG-161
浏览量:127
品牌:Excellgen
服务
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商品描述
Description

T4EndonucleaseV (T4pyrimidinedimerglycosylase, T4PDG),hasbothDNAN-glycosylaseandapurinic/apyrimidiniclyase(APlyase)activities.Thisenzyme recognizes cis-syn-cyclobutanepyrimidinedimersinDNAcausedbyultraviolet(UV)irrADIation,andtheendonucleolyticactivitycleavesthephosphodiesterbondattheAPsite.

Applications
  • StudyofUVdamagetoDNAanditsrepair,includingDNAdamageinsinglecells
  • Singlecellgelelectrophoresis(cometassay)
SourcePurifiedfromanE.colistraincarryingaplasmidencodingT4denVgene.
Components

T4EndonucleaseV:10,000units/mlin10mMTris-HCl,100mMNaCl,0.1mMDTT,0.1mMEDTA,50%Glycerol,pH7.5@25°C

UnitDefinition

OneunitofT4EndonucleaseVconverts1µgofUV-irradiatedplasmidDNAfromcovalentlyclosedcirclestonickedformin30minutesat37°C.
QualityControl

Theabsenceofnonspecificexo-andendonucleaseactivitiesandRNaseactivityisconfirmedbyappropriatequalitytest.

StorageCondition

-20°C

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