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Excellgen/T4 Phage beta-glucosyltransferase (T4-BGT)/EG-158/2,500 Units

价格
¥6360.00
货号:EG-158-2,500Units
浏览量:118
品牌:Excellgen
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商品描述
Description

T4Phageβ-glucosyltransferaseisaDNA-modifyingenzymeencodedbybacteriophageT4thatspecificallytransferstheglucosemoietyofuridinediphosphoglucose(UDP-Glc)tothe5-hydroxymethylcytosine(5-hmC)residuesindouble-strandedDNA,resultingintheformationofbeta-glucosyl-5-hydroxymethylcytosine.

Applications
  • Locusspecificimmunodetectiondetectionof5-hmCDNA
  • Enrichmentof5-hmCcontainingDNA
  • Labelingof5-hmCresiduesusingarADIoactiveUDP-glucosedonor
SourceAnE.colistrainthatcarriestheclonedbgtgenefrombacteriophageT4
UnitDefinition

OneunitistheamountofT4Beta-glucosyltransferaserequiredtoprotect0.5µgoffully5-hydroxymethylated1kbPCRfragmentfromcleavagebyEcoR1.

Components
  • T4Beta-glucosyltransferase:10,000units/mlin10mMTris(pH7.5at25°C),500mMNaCl,0.1mMEDTA,1mMDTTand50%(v/v)glycerol.
  • 10XReactionBuffer:330mMTris-acetate(pH7.5),660mMpotassiumacetate,100mMmagnesiumacetate,and5mMDTT.
QualityControl

Theabsenceofexonuclease,endonuclease,andRNaseisconfirmedbyappropriatequalitytest.

StorageCondition

-20°C

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