Description | T4Phageβ-glucosyltransferaseisaDNA-modifyingenzymeencodedbybacteriophageT4thatspecificallytransferstheglucosemoietyofuridinediphosphoglucose(UDP-Glc)tothe5-hydroxymethylcytosine(5-hmC)residuesindouble-strandedDNA,resultingintheformationofbeta-glucosyl-5-hydroxymethylcytosine. |
Applications | - Locusspecificimmunodetectiondetectionof5-hmCDNA
- Enrichmentof5-hmCcontainingDNA
- Labelingof5-hmCresiduesusingarADIoactiveUDP-glucosedonor
|
Source | AnE.colistrainthatcarriestheclonedbgtgenefrombacteriophageT4 |
UnitDefinition | OneunitistheamountofT4Beta-glucosyltransferaserequiredtoprotect0.5µgoffully5-hydroxymethylated1kbPCRfragmentfromcleavagebyEcoR1. |
Components | - T4Beta-glucosyltransferase:10,000units/mlin10mMTris(pH7.5at25°C),500mMNaCl,0.1mMEDTA,1mMDTTand50%(v/v)glycerol.
- 10XReactionBuffer:330mMTris-acetate(pH7.5),660mMpotassiumacetate,100mMmagnesiumacetate,and5mMDTT.
|
QualityControl | Theabsenceofexonuclease,endonuclease,andRNaseisconfirmedbyappropriatequalitytest. |
StorageCondition | -20°C |
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