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蚂蚁淘在线 / 品牌中心 / Excellgen / Excellgen/E. coli Formamidopyrimidine-DNA glycosylase, fpg, Mut M/EG-1006/500 Units

Excellgen/E. coli Formamidopyrimidine-DNA glycosylase, fpg, Mut M/EG-1006/500 Units

价格
￥1000.00
货号：EG-1006-500Units
浏览量：127
品牌：Excellgen
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商品描述
Description

Fpg(alsoknownasformamidopyrimidine[fapy]-DNAglycosylase,MutM,FAPYDNAGlycosylase,and8-oxoguanineDNAglycosylase)hasbothN-glycosylaseandAP-lyaseactivities.TheN-glycosylaseactivityreleasesdamagedpurinesfromdoublestrandedDNA,generatinganapurinic(APsite).TheAP-lyaseactivitycleavesboth3´and5´totheAPsitetherebyremovingtheAPsiteandproducinga1basegapintheDNAand3′and5′phosphatetermini.BasesrecognizedandremovedbyFpginclude7,8-dihydro-8-oxoguanine(8-oxoguanine),8-oxoadenine,fapy-guanine,methy-fapy-guanine,fapy-adenine,aflatoxinB1-fapy-guanine,5-hydroxy-cytosineand5-hydroxy-uracil(1,2).

Applications
  • DNANicking
  • DNARepair
  • NickTranslation
SourceAnE.colistrainthatcarriestheclonedfpggene.
UnitDefinitionOneunitisdefinedastheamountofenzymerequiredtocleave1pmolofa34-meroligonucleotideduplexcontainingasingle8-oxoguaninebasepairedwithacytosineinatotalreactionvolumeof10μlin1hourat37°C
Components
  • E.colifpg:8,000U/mlin20mMTris-HCl,pH8.0@25°C,50mMNaCl,0.5mMEDTA,200μg/mlBSA,50%Glycerol
  • 10XReactionBuffer:100mMBis-Tris-Propane,100mMMgCl2,10mMDTT,pH7.0@25°C
QualityControlTheabsenceofendo-,exodeoxyribonucleasesandribonucleasesconfirmedbyappropriatequalitytests.
StorageCondition

-20°C

References
  1. Tchou,J.etal.(1994)SubstratespecificityofFpgprotein.J.Biol.Chem.,269,15318-15324.
  2. Hatahet,Z.etal.(1994)Newsubstratesforoldenzymes.J.Biol.Chem.,269,18814-18820.
  3. Boiteux,S.,O’Connor,T.andLaval,J.(1987)Formamidopyrimidine-DNAglycosylaseofEscherichiacoli:cloningandsequencingofthefpgstructuralgeneandoverproductionoftheprotein.EMBOJ.,5,3177-3183.
  4. Singh,N.,McCoy,M.,Tice,R.andSchneider,L.(1988)AsimpletechniqueforquantitationoflowlevelsofDNAdamageinindividualcells.Exp.CellRes.,175,184-191.
  5. Collins,A.,Duthie,S.andDobson,V.(1993)DirectenzymaticdetectionofendogenousoxidativebasedamageinhumanlymphocyteDNA.Carcinogenesis,14,1733-1735.
  6. Collins,A.,Dusinska,M.,Gedik,C.andStetina,R.(1996)OxidativedamagetoDNA:dowehaveareliablebioMarker?.Environ.HealthPerspect.,104,465-469.
  7. Pflaum,M.,Will,O.,Mahler,H.-C.andEpe,B.(1998)DNAoxidationproductsdeterminedwithrepairendonucleasesinmammaliancells:types,basallevelsandinfluenceofcellproliferation.FreeRad.Res.,29,585-594.
  8. Hartwig,A.,Dally,H.andSchlepegrell,R.(1996)SensitiveanalysisofoxidativeDNAdamageinmammaliancells:useofthebacterialFpgproteinincombinationwithalkalineunwinding.Toxicol.Lett.,88,85-90.
  9. Czene,S.andHarms-Ringdahl,M.(1995)Detectionofsinglestrandbreaksandformamidopyrimidine-DNAglycosylase-sensitivesitesinDNAofculturedhumanfibroblasts.Mutat.Res.,336,235-242.
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