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Description | T4PolynucleotideKinase(PNK)catalyzesthetransferoftheterminalgamma-phosphatefromATPtothe5′-OHgroupofsingle-anddouble-strandedDNA,RNA,oligonucleotidesandnucleoside3′-monophosphatemolecules.InthepresenceofADPT4PolynucleotideKinasealsoexhibits5'-phosphataseactivityandcatalyzestheexchangeofphosphategroupsbetween5'-P-oligo-polynucleotidesandATP. |
Applications |
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Source | ArecombinantE.colistraincarryingT4phagepseTgeneforpolynucleotidekinase. |
UnitDefinition | Oneunitisdefinedastheamountofenzymecatalyzingtheincorporationof1nmolof[32P](ATPdonor)in30minutesat37°Cin1XT4PolynucleotideKinaseReactionBuffer. |
Components |
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QualityControl | Theabsenceofendo-,exodeoxyribonucleasesandribonucleasesconfirmedbyappropriatequalitytests.Functionallytestedforlabeling5'-terminiofDNA. |
StorageCondition | -20°C |