品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
| Description | T4PolynucleotideKinase(PNK)catalyzesthetransferoftheterminalgamma-phosphatefromATPtothe5′-OHgroupofsingle-anddouble-strandedDNA,RNA,oligonucleotidesandnucleoside3′-monophosphatemolecules.InthepresenceofADPT4PolynucleotideKinasealsoexhibits5'-phosphataseactivityandcatalyzestheexchangeofphosphategroupsbetween5'-P-oligo-polynucleotidesandATP. |
| Applications |
|
| Source | ArecombinantE.colistraincarryingT4phagepseTgeneforpolynucleotidekinase. |
| UnitDefinition | Oneunitisdefinedastheamountofenzymecatalyzingtheincorporationof1nmolof[32P](ATPdonor)in30minutesat37°Cin1XT4PolynucleotideKinaseReactionBuffer. |
| Components |
|
| QualityControl | Theabsenceofendo-,exodeoxyribonucleasesandribonucleasesconfirmedbyappropriatequalitytests.Functionallytestedforlabeling5'-terminiofDNA. |
| StorageCondition | -20°C |


