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蚂蚁淘在线 / 品牌中心 / Excellgen / Excellgen/Thermus thermophilus Muts DNA mismatch repair protein/EG-198/500 µg

Excellgen/Thermus thermophilus Muts DNA mismatch repair protein/EG-198/500 µg

价格
￥7900.00
货号：EG-198
浏览量：127
品牌：Excellgen
服务
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商品描述
ProductNameThermusThermophilusMutSDNAmismatchrepairprotein
Size500µg
Description

TheTthMutSDNAmismatchproteinrecognizesheteroduplexDNAscontainingmispairedorunpairedbases.This91.8-kDaproteinbindsinvitrotoheteroduplexDNAscontainingmispairedorunpairedbasesoverawidetemperaturerangefrom4to70 °CandhasathermostableATPaseactivity.ThisthermostableTthMutSisactiveattemperaturebetween0to75°C.SinceTthMutSefficientlybindsto1-4basesdeletion(orinsertion)andmismatchbasepairsofGT,CTandAG,itisusefulfordetectingthesemutations.MutationscanbedetectedinpolyacrylamidegelsoronasolidphasesuchasNiagaroseormagneticNi-NTAparticles.

Applications
  • RemovemismatchDNA(errorcorrection)fromgenesynthesisreaction
  • Mutationdetectionandremoval
  • RapidisothermalSNPdetection
SourceE.coli
FusionTag6XHistagatN-terminus
PurificationMethodFPLC
Concentration1mg/ml
Purity

~95%asdeterminedbySDS-PAGE

Accession#WP_011173381
GeneName

ThermusthermophilusMutSDNAmismatchrepairprotein

MW135.9kDa
ProteinSequence1MGSSHHHHHHMGNMLKGEGPGPLPPLLQQYVELRDRYPDYLLLFQVGDFYECFGEDAERL
61ARALGLVLTHKTSKDFTTPMAGIPIRAFDAYAERLLKMGFRLAVADQVEPAEEAEGLVRR
121EVTQLLTPGTLTQEALLPREANYLAAIATGDGWGLAFLDVSTGEFKGTLLKSKSALYDEL
181FRHRPAEVLLAPELRENEAFVAEFRKRFPVMLSEAPFEPQGEGPLALRRAQGALLAYARA
241TQGGALSVRPFRLYDPGAFVRLPEASLKALEVFEPLRGQDTLFGVLDETRTAPGRRLLQA
301WLRHPLLERGPLEARLDRVERFVREGALREGVRRLLFRLADLERLATRLELSRASPRDLA
361ALRRSLEILPELKGLLGEEVGLPDLSGLLEELRAALVEDPPLKVSEGGLIREGYDPDLDA
421LRRAHAEGVAYFLDLEAREKERTGIPTLKVGYNAVFGYYLEVTRPYYEKVPQEYRPVQTL
481KDRQRYTLPEMKERERELYRLEALIKRREEEVFLALRERARKEAEALREAARILAELDVY
541AALAEVAVRHGYTRPRFGERLRIRAGRHPVVERRTAFVPNDLEMAHELVLVTGPNMAGKS
601TFLRQTALIALLAQIGSFVPAEEAELPLFDGIYTRIGASDDLAGGKSTFMVEMEEVALVL
661KEATERSLVLLDEVGRGTSSLDGVAIATALAEALHERRCYTLFATHYFELTALALPRLKN
721LHVAAKEEEGGLVFYHQVLPGPASKSYGVEVAEMAGLPKEVVERARALLSAMAARREGAL
781EEVLERLLALDPDRLTPLEALRFLHELKALALGLPLGSMKG
StorageBuffer20mMTris-HCl,pH8.0,250mMNaCl,0.1mMEDTA,1mMDTT,50%Glycerol
ReactionBuffer100mMKCl,50mMTris-HCl,pH8.5,5~20mMMgCl2,0.1mMEDTA,1mMDTT,2%Glycerol,65°C
Storage-20to-80°C.
Shipping4°Cordryice
References

1.Protein-mediatederrorcorrectionfordenovoDNAsynthesis.NucleicAcidsRes.2004Nov23;32(20):e162.

2.CorrectingerrorsinsyntheticDNAthroughconsensusshuffling.NucleicAcidsRes.2005Mar30;33(6):e55.

3.MutSasatoolformutationdetection.ActaBiochimPol.2005;52(3):575-83.Epub2005Aug4.

4.OnetubemutationdetectionusingsensitivefluorescentdyeingofMutSprotectedDNA.NucleicAcidsRes.2000Apr15;28(8):E36.

5.RapidSNPdiagnosticsusingasymmetricisothermalamplificationandanewmismatch-suppressiontechnology.NatureMethods-4,257-262(2007)doi:10.1038/nmeth1007

Excellgen蛋白表达产品名称-目录 ＃价钱293Expresso™转染试剂EG-1089$ 98.00定制抗体生产和免疫测定开发EG-36从培养基中纯化IgEEG-1076-1$ 750.00MembraneExpress TM功能膜蛋白的病毒样颗粒EG-1038蛋白质表达和纯化服务EG-1076-8$ 1,500.00小规模表达测试和纯化方法开发EG-1081-3$ 1,250.00使用HEK 293细胞的瞬时蛋白质生产EG-1080$ 2,900.00  $ 1,950.00-$ 950.00使用细菌表达系统进行蛋白表达和纯化EG-1076$ 2,170.00使用杆状病毒表达系统进行蛋白质表达和纯化EG-1078$ 4,500.00使用Akta FPLC系统的定制蛋白质纯化服务EG-1082$ 1,000.00使用稳定的哺乳动物细胞系表达蛋白质EG-1081$ 9,500.00