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蚂蚁淘在线 / 品牌中心 / Excellgen / Excellgen/Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts/EG-41/500 µg

Excellgen/Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts/EG-41/500 µg

价格
￥38000.00
货号：EG-41-500µg
浏览量：127
品牌：Excellgen
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商品描述
ProductNameThermusaquaticusMutSDNAmismatchrepairprotein,6XHis-Taq-Muts
Size500µg
Description

TheTaqMutSDNAmismatchproteinrecognizesheteroduplexDNAscontainingmispairedorunpairedbases.ThisMutsproteinbindsinvitrotoheteroduplexDNAscontainingmispairedorunpairedbasesoverawidetemperaturerangefrom4to70 °CandhasaThermostableATPaseactivity.ThisthermostableTaqMutSisactiveattemperaturebetween0to75°C.SinceTaqMutSefficientlybindsto1-4basesdeletion(orinsertion)andmismatchbasepairsofGT,CTandAG,itisusefulfordetectingthesemutations.MutationscanbedetectedinpolyacrylamidegelsoronasolidphasesuchasNiagaroseorbeadsormagneticNi-NTAparticles.

Applications
  • RemovemismatchDNA(errorcorrection)fromgenesynthesisreaction
  • Mutationdetectionandremoval
  • RapidisothermalSNPdetection
SourceE.coli
FusionTag6XHistagatC-terminus
PurificationMethodFPLC
Concentration1mg/ml
Purity

~95%asdeterminedbySDS-PAGE

Accession#AAC43637,TAU3311,U33117
GeneNameThermusaquaticusMutSDNAmismatchrepairprotein
MW92.8kDa
ProteinSequence

MKIEHMEGMLKGEGPGPLPPLLQQYVELRDQYPDYLLLFQVGDFYECFGEDAERLARALG
LVLTHKTSKDFTTPMAGIPLRAFEAYAERLLKMGFRLAVADQVEPAEEAEGLVRREVTQL
LTPGTLLQESLLPREANYLAAIATGDGWGLAFLDVSTGEFKGTVLKSKSALYDELFRHRP
AEVLLAPELLENGAFLDEFRKRFPVMLSEAPFEPEGEGPLALRRARGALLAYAQRTQGGA
LSLQPFRFYDPGAFMRLPEATLRALEVFEPLRGQDTLFSVLDETRTAPGRRLLQSWLRHP
LLDRGPLEARLDRVEGFVREGALREGVRRLLYRLADLERLATRLELGRASPKDLGALRRS
LQILPELRALLGEEVGLPDLSPLKEELEAALVEDPPLKVSEGGLIREGYDPDLDALRAAH
REGVAYFLELEERERERTGIPTLKVGYNAVFGYYLEVTRPYYERVPKEYRPVQTLKDRQR
YTLPEMKEKEREVYRLEALIRRREEEVFLEVRERAKRQAEALREAARILAELDVYAALAE
VAVRYGYVRPRFGDRLQIRAGRHPVVERRTEFVPNDLEMAHELVLITGPNMAGKSTFLRQ
TALIALLAQVGSFVPAEEAHLPLFDGIYTRIGASDDLAGGKSTFMVEMEEVALILKEATE
NSLVLLDEVGRGTSSLDGVAIATAVAEALHERRAYTLFATHYFELTALGLPRLKNLHVAA
REEAGGLVFYHQVLPGPASKSYGVEVAAMAGLPKEVVARARALLQAMAARREGALDAVLE
RLLALDPDRLTPLEALRLLQELKALALGAPLDTMKGKLAAALEHHHHHH

StorageBuffer20mMTris-HCl,pH8.0,250mMNaCl,0.1mMEDTA,1mMDTT,50%Glycerol
ReactionBuffer100mMKCl,50mMTris-HCl,pH8.5,5~20mMMgCl2,0.1mMEDTA,1mMDTT,2%Glycerol,65°C
Storage-20to-80°C.
Shipping4°Cordryice

Protocol

(example)

1.AfterfirstroundPCR,purifyPCRfragmentsusingQiagenQIAquickPCRpurificationkitwithelutionindH2O.

2.DilutePCRproductto250ng/µlin10mMTris–HCl,pH7.8,50mMNaClandheatto95oCfor5minfollowedbycoolingat0.1oC/sto25oC.

3.Addbindingbuffer(20mMTris–HCl,pH7.8,10mMNaCl,5mMMgCl2,1mMDTTand5%glycerol)toannealedPCRproduct,adjustDNAconcentrationto11.5ng/µl,add6XHis-MutSdimersto950nM.

4.Incubatethemixtureatroomtemperaturefor10min,thenaddanequalvolumeofNi-NTAbeadspreequilibratedwith1Xbindingbuffer,andincubatefor30minatroomtemperature.

5.Gentlyspindownbeadsandsavesupernatantforsubsequentprocessing(secondroundPCR,cloningetc).

References

1.Protein-mediatederrorcorrectionfordenovoDNAsynthesis.NucleicAcidsRes.2004Nov23;32(20):e162.

2.CorrectingerrorsinsyntheticDNAthroughconsensusshuffling.NucleicAcidsRes.2005Mar30;33(6):e55.

3.MutSasatoolformutationdetection.ActaBiochimPol.2005;52(3):575-83.Epub2005Aug4.

4.OnetubemutationdetectionusingsensitivefluorescentdyeingofMutSprotectedDNA.NucleicAcidsRes.2000Apr15;28(8):E36.

5.RapidSNPdiagnosticsusingasymmetricisothermalamplificationandanewmismatch-suppressiontechnology.NatureMethods-4,257-262(2007)doi:10.1038/nmeth1007

Excellgen蛋白表达产品名称-目录 ＃价钱293Expresso™转染试剂EG-1089$ 98.00定制抗体生产和免疫测定开发EG-36从培养基中纯化IgEEG-1076-1$ 750.00MembraneExpress TM功能膜蛋白的病毒样颗粒EG-1038蛋白质表达和纯化服务EG-1076-8$ 1,500.00小规模表达测试和纯化方法开发EG-1081-3$ 1,250.00使用HEK 293细胞的瞬时蛋白质生产EG-1080$ 2,900.00  $ 1,950.00-$ 950.00使用细菌表达系统进行蛋白表达和纯化EG-1076$ 2,170.00使用杆状病毒表达系统进行蛋白质表达和纯化EG-1078$ 4,500.00使用Akta FPLC系统的定制蛋白质纯化服务EG-1082$ 1,000.00使用稳定的哺乳动物细胞系表达蛋白质EG-1081$ 9,500.00