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蚂蚁淘在线 / 品牌中心 / Excellgen / Excellgen/Thermus aquaticus Muts DNA mismatch repair protein, Taq-Muts, no tag/EG-25/500 µg

Excellgen/Thermus aquaticus Muts DNA mismatch repair protein, Taq-Muts, no tag/EG-25/500 µg

价格
￥43900.00
货号：EG-25-500µg
浏览量：127
品牌：Excellgen
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商品描述
ProductNameThermusaquaticusMutSDNAmismatchrepairprotein
DescriptionTheTaqMutSDNAmismatchproteinrecognizesheteroduplexDNAscontainingmispairedorunpairedbases.ThisMutsproteinbindsinvitrotoheteroduplexDNAscontainingmispairedorunpairedbasesoverawidetemperaturerangefrom4to70 °CandhasaThermostableATPaseactivity.ThisthermostableTaqMutSisactiveattemperaturebetween0to75°C.SinceTaqMutSefficientlybindsto1-4basesdeletion(orinsertion)andmismatchbasepairsofGT,CTandAG,itisusefulfordetectingthesemutations.Mutationscanbedetectedinpolyacrylamidegels
Applications
  • RemovemismatchDNA(errorcorrection)fromgenesynthesisreaction
  • Mutationdetectionandremoval
  • RapidisothermalSNPdetection
SourceE.coli
FusionTagNone
PurificationMethodFPLC
Concentration1mg/ml
Purity

~95%asdeterminedbySDS-PAGE

Accession#AAC43637,TAU3311,U33117
GeneNameThermusaquaticusMutSDNAmismatchrepairprotein
ProteinSequence1MEGMLKGEGPGPLPPLLQQYVELRDQYPDYLLLFQVGDFYECFGEDAERLARALGLVLTH
61KTSKDFTTPMAGIPLRAFEAYAERLLKMGFRLAVADQVEPAEEAEGLVRREVTQLLTPGT
121LLQESLLPREANYLAAIATGDGWGLAFLDVSTGEFKGTVLKSKSALYDELFRHRPAEVLL
181APELLENGAFLDEFRKRFPVMLSEAPFEPEGEGPLALRRARGALLAYAQRTQGGALSLQP
241FRFYDPGAFMRLPEATLRALEVFEPLRGQDTLFSVLDETRTAPGRRLLQSWLRHPLLDRG
301PLEARLDRVEGFVREGALREGVRRLLYRLADLERLATRLELGRASPKDLGALRRSLQILP
361ELRALLGEEVGLPDLSPLKEELEAALVEDPPLKVSEGGLIREGYDPDLDALRAAHREGVA
421YFLELEERERERTGIPTLKVGYNAVFGYYLEVTRPYYERVPKEYRPVQTLKDRQRYTLPE
481MKEKEREVYRLEALIRRREEEVFLEVRERAKRQAEALREAARILAELDVYAALAEVAVRY
541GYVRPRFGDRLQIRAGRHPVVERRTEFVPNDLEMAHELVLITGPNMAGKSTFLRQTALIA
601LLAQVGSFVPAEEAHLPLFDGIYTRIGASDDLAGGKSTFMVEMEEVALILKEATENSLVL
661LDEVGRGTSSLDGVAIATAVAEALHERRAYTLFATHYFELTALGLPRLKNLHVAAREEAG
721GLVFYHQVLPGPASKSYGVEVAAMAGLPKEVVARARALLQAMAARREGALDAVLERLLAL
781DPDRLTPLEALRLLQELKALALGAPLDTMKG
StorageBuffer20mMTris-HCl,pH8.0,250mMNaCl,1mMDTT,50%Glycerol
ReactionBuffer100mMKCl,50mMTris-HCl,pH8.5,5~20mMMgCl2,0.1mMEDTA,1mMDTT,2%Glycerol,65°C
Storage-20to-80°C.
Shipping4°Cordryice
References
  1. Protein-mediatederrorcorrectionfordenovoDNAsynthesis.NucleicAcidsRes.2004Nov23;32(20):e162.
  2. CorrectingerrorsinsyntheticDNAthroughconsensusshuffling.NucleicAcidsRes.2005Mar30;33(6):e55.
  3. MutSasatoolformutationdetection.ActaBiochimPol.2005;52(3):575-83.Epub2005Aug4.
  4. OnetubemutationdetectionusingsensitivefluorescentdyeingofMutSprotectedDNA.NucleicAcidsRes.2000Apr15;28(8):E36.
  5. RapidSNPdiagnosticsusingasymmetricisothermalamplificationandanewmismatch-suppressiontechnology.NatureMethods-4,257-262(2007)doi:10.1038/nmeth1007
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