Size | 100µg |
Background | Atleast23differentvariantsofIFN-alphaareknown.Theindividualproteinshavemolecularmassesbetween19-26kDaandconsistofproteinswithlengthsof156-166and172aminoacids.AllIFN-alphasubtypespossessacommonconservedsequenceregionbetweenaminoacidpositions115-151whiletheamino-terminalendsarevariable.ManyIFN-alphasubtypesdifferintheirsequencesatonlyoneortwopositions.Naturallyoccurringvariantsalsoincludeproteinstruncatedby10aminoacidsatthecarboxy-terminalend. |
Description | RecombinantHumanIFN-alpha2aproducedinE.coliisasingle,non-glycosylated,polypeptidechaincontaining165aminoacidsandhasamolecularmassof19241Dalton. |
BIOLOGicalactivity | ThespecificactivityasdeterminedinaviralresistanceassayusingVSV-WISHcellswasfoundtobegreaterthan1.4x108IU/mg |
Purity |
(a)AnalysisbySEC-HPLC. (b)Analysisbyreducingandnon-reducingSDS-PAGESilverStainedgel.
|
Molecularweigh | 19.2KD+/-10%determinedbyreducedSDS-PAGE |
Amino-AcidSequence | ThesequenceofthefirstfifteenN-terminalaminoacidswasdeterminedandwasfoundtobeMet-Cys-Asp-Leu-Pro-Gln-Thr-His-Ser-Leu-Gly-Ser-Arg-Arg-Thr-Leu. |
Formulation | 50mMacetatepH4.8.sterilefiltered |
Storage | -20ot-80°C. |
Shipping | 4°Cordryice |
Note | Forresearchuseonly |