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商品描述
| ProductName | TNF-alpha(LowEndotoxin) |
| Size | 10µg |
| Description | Tumornecrosisfactoralpha(TNFαorTNFSF2)isproducedbymanydifferentcelltypesincludingactivatedmonocytes,fibroblasts,endothelialcells,macrophages,T-cells,B-lymphocytes,granulocytes,smoothmusclecells,eosinophilis,chondrocytesandothercelltypes(1).Itfunctionsasacytokineinvolvedincellproliferation,differentiation,apoptosis,lipidmetabolismandcoagulation(2,3).HighlevelofTNFαleadstothedevelopmentofinflammatoryresponsesthatarehallmarksofmanydiseasesincludingautoimmunediseases,insulinresistanceandcancer(4,5).TheactiveformofTNFa(18kDa)isgeneratedbythecleavageofanAla-Valbondbetweenresidues76-77oftheprecursorprotein(6). |
| Applications | cytotoxicityassaysandothercellassays |
| Source | E.coli |
| FusionTag | 6XHis |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogramofpurifiedprotein. |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 7124 |
| Accession# | NM_000594 |
| GeneName | tumornecrosisfactor |
| GeneSymbol | TNF |
| GeneSynonyms | DIF;TNFA;TNFSF2;TNF-alpha |
| MW | 21kDa |
| ProteinSequence | VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYS QVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYL GGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL |
| StorageBuffer | 20mMTris-HClpH7.9,100mMNaCl,0.2mMEDTA,1mMDTTand20%glycerol |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Locksley,R.etal.,(2001)Cell104,487-501 2.Mukhopadhyay,S.etal.,(2006)RespiratoryRes.7,1-9 3.Aggarwal,B.(2003)Nat.Rev.Immunol.3,745-756 4.Walczak,H.etal.,(1999)Nat.Med.5,157-163 5.Lam,J.etal.,(2000)J.Clin.Invest.106,1481-1488 6.Mcgeehan,G.etal.,(1994)Nature370,558-561 |
