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蚂蚁淘在线 / 品牌中心 / Excellgen / Excellgen/Nuclear factor-like 2, NRF2, NFE2L2, nuclear factor erythroid 2-related factor 2/RP-123/100

Excellgen/Nuclear factor-like 2, NRF2, NFE2L2, nuclear factor erythroid 2-related factor 2/RP-123/100

价格
￥5000.00
货号：RP-123
浏览量：127
品牌：Excellgen
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商品描述
ProductNameNuclearfactor-like2,NRF2,NFE2L2
Size100µg
DescriptionNuclearfactor(erythroid-derived2)-like2,alsoknownasNFE2L2orNrf2,isatranscriptionfactorthatbindstotheantioxidantresponseelement(ARE)oftargetgenesinresponsetooxidativestressandincreasesthetranscriptionofavarietyofantioxidativeandcarcinogen-detoxificationenzymes.Stresscanresultfromavarietyofcauses,includingfasting,overfeeding,endogenouscompounds,exposuretochemicals,orenvironmentalagents,whichgenerallyleadstoincreasedproductionofreactiveoxygenspecies(ROS).Undernormalconditions,Nrf2isboundtoKelchlike-ECH-associatedprotein1(Keap1).Keap1actsasasubstrateadaptorproteinforCullin3-basedubiquitination,whichresultsinthedegradationofNrf2,andundernormalconditionsNrf2hasahalf-lifeofonly20minutes.OxidativestressorelectrophilicstressdisruptscriticalcysteineresiduesinKeap1,resultinginadisruptionoftheKeap1-Cul3ubiquitinationsystemandabuild-upofNrf2inthecytoplasmandtranslocatestothenucleus,whereitbindstoAREstoinduceexpressionofmultiplecytoprotectiveenzymes,includingNAD(P)H-quinoneoxidoreductase1(NQO1),glutathioneS-transferases(GSTs),andhemeoxygenase1(HO-1)(1,2).
Nrf2alsoinducesseveraltranscriptionfactorsinvolvedinmitochondrialbiogenesis.ManylinesofevidencesuggestthatmitochondrialdysfunctionplaysacentralroleinthepathogenesisofPD.Thus,Nrf2maybeapromisingtargettocounteractmitochondrialdysfunctionanditsconsequencesinParkinson'sdisease(PD)(3).TheKeap1–Nrf2regulatorysystemisadouble-edgedswordforourbody.High-levelNrf2activityincreasescellprotectionandmakesthecellresistanttooxidativeandelectrophilicstresses.Therefore,Nrf2inducerscouldbeeffectiveforcancerchemoprevention.Ontheotherhand,elevatedNrf2activityisalsoadvantageousforcancercellsurvivalandevolution.Thus,Nrf2inhibitorscouldbeeffectiveforanticancertherapy.(4)
ApplicationsDNA-protein,protein-proteininteractionresearchandassaydevelopment.
SourceE.coli
FusionTag6XHis
PurificationMethodFPLC
Purity

~95%asdeterminedbySDS-PAGE

GeneID4780
Accession#NP_006155,NM_006164
GeneNameNuclearfactor(erythroid-derived2)-like2
GeneSymbolNFE2L2
GeneSynonymsNRF2
MW69.9kDa
ProteinSequenceMMDLELPPPGLPSQQDMDLIDILWRQDIDLGVSREVFDFSQRRKEYELEKQKKLEKERQE
QLQKEQEKAFFAQLQLDEETGEFLPIQPAQHIQSETSGSANYSQVAHIPKSDALYFDDCM
QLLAQTFPFVDDNEVSSATFQSLVPDIPGHIESPVFIATNQAQSPETSVAQVAPVDLDGM
QQDIEQVWEELLSIPELQCLNIENDKLVETTMVPSPEAKLTEVDNYHFYSSIPSMEKEVG
NCSPHFLNAFEDSFSSILSTEDPNQLTVNSLNSDATVNTDFGDEFYSAFIAEPSISNSMP
SPATLSHSLSELLNGPIDVSDLSLCKAFNQNHPESTAEFNDSDSGISLNTSPSVASPEHS
VESSSYGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSSGDMVQPLSPSQGQSTHVHDA
QCENTPEKELPVSPGHRKTPFTKDKHSSRLEAHLTRDELRAKALHIPFPVEKIINLPVVD
FNEMMSKEQFNEAQLALIRDIRRRGKNKVAAQNCRKRKLENIVELEQDLDHLKDEKEKLL
KEKGENDKSLHLLKKQLSTLYLEVFSMLRDEDGKPYSPSEYSLQQTRDGNVFLVPKSKKP
DVKKN
StorageBuffer20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA
Storage-20to-80°C.
Shipping4°Cordryice
References

1.IshiiT.,ItohK.,YamamotoM(2002)RolesofNrf2inactivationofantioxidantenzymegenesviaantioxidantresponsiveelements.MethodsEnzymol348:182–190.
2.KobayashiM.,YamamotoM(2006)Nrf2-Keap1regulationofcellulardefensemechanismsagainstelectrophilesandreactiveoxygenspecies.AdvEnzymeRegul46:113–140.
3.TufekciK.,GencK.,et.al(2011)TheNrf2/AREPathway:APromisingTargettoCounteractMitochondrialDysfunctioninParkinson'sDisease.Parkinson'sDisease314082
4.TaguchiK.,YamamotoM.,et.al(2011)MolecularmechanismsoftheKeap1–Nrf2pathwayinstressresponseandcancerevolutionGenestoCells16,123–140.
5.LeeJM.andJohnsonJ.,(2004)AnImportantRoleofNrf2-AREPathwayintheCellularDefenseMechanism.JournalofBiochemistryandMolecularBIOLOGy37,139-143.

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