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苏州工业园区生物纳米园A4#216
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商品描述
| ProductName | c-fos(protooncogene) |
| Size | 5,000U |
| Description | ThetranscriptionfactorAP-1(activatorprotein-1)isinvolvedincellularproliferation,transformationanddeath.AP-1andnuclearfactorB(NFkB)canbespecificallytargetedtopreventcancerinductioninmousemodels.AP-1canbeproducedby18differentdimericcombinationsofproteinsfromtheJun[c-Jun(cat#P2014),JunBandJunD]andFos(c-Fos,FosB,Fra-1andFra-2)families,includingJunhomodimersandJun–Fosheterodimers(1–4).TheJunandFosproteinscontainabasic-regionleucinezipper(bZIP)domain,andarecapableofbindingtootherbZIPproteinsincludingthosefromtheATF,MAF,CNCandC/EBP(CCAAT/enhancer-bindingprotein)subfamilies(5).Jun–JunandJun–Fosdimersbindwithhighestaffinitytothe12-O-tetradecanoylphorbol-13-acetate(TPA)responseelement(TRE)[TGA(C/G)TCA](6),althoughmanyotherAP-1-likesiteshavebeenreported.Bindingtoanyofthesesitescanbetissue-specific,oraffectedbyneighboringsequences,anddependentuponinteractionswithothertranscriptionfactorsorcofactors.JunandFosproteinscanalsodimerizewithotherbZIPproteins,allowingthemtotargetotherDNAbindingsites,suchasthecAMPresponseelement(CRE),theantioxidantresponseelements(ARE),andhalf-sitescomposedofhalfofaTREsiteandhalfofaMAF-orCNC-bindingsite.Inaddition,AP-1proteinscaninteractwithotherproteins,includingthep65subunitofNF-B,CBP(CRE-binding-protein-bindingprotein)(p300),SMAD-3and-4,andtheretinoblastomaprotein(see5foramorecompletelist),furtherincreasingthecombinatorialpotentialofJunandFosproteins.AP-1regulatesavarietyofcellularprocesses,includingproliferation,differentiationandapoptosis,andcontributestobothbasalandstimulus-activatedgeneexpression.Itisactivatedbygrowthfactors,hormones,stress,cytokines,ROSandultravioletrADIation.Activationoccursbothtranscriptionallyandpost-translationally,andissignaledpredominantlythroughthemitogen-activatedproteinkinase(MAPK)cascade(7).ThecombinatorialdiversityofAP-1proteinsandotherinteractingfactorsappearstoinfluencehowspecificcelltypesrespondtoastimulus. |
| Applications | protein-DNAandprotein-proteininteractionassays |
| Source | Baculovirus-insectcell |
| FusionTag | 6XHis |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogramofpurifiedprotein.100unitsaresufficientforaprotein-proteininteractionassay. |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 6722 |
| Accession# | NM_003131 |
| GeneName | serumresponsefactor(c-fosserumresponseelement-bindingtranscriptionfactor) |
| GeneSymbol | SRF |
| GeneSynonyms | MCM1 |
| MW | 53.3kDa |
| ProteinSequence | MLPTQAGAAAALGRGSALGGSLNRTPTGRPGGGGGTRGANGGRVPGNGAGLGPGRLEREA AAAAATTPAPTAGALYSGSEGDSESGEEEELGAERRGLKRSLSEMEIGMVVGGPEASAAA TGGYGPVSGAVSGAKPGKKTRGRVKIKMEFIDNKLRRYTTFSKRKTGIMKKAYELSTLTG TQVLLLVASETGHVYTFATRKLQPMITSETGKALIQTCLNSPDSPPRSDPTTDQRMSATG FEETDLTYQVSESDSSGETKDTLKPAFTVTNLPGTTSTIQTAPSTSTTMQVSSGPSFPIT NYLAPVSASVSPSAVSSANGTVLKSTGSGPVSSGGLMQLPTSFTLMPGGAVAQQVPVQAI QVHQAPQQASPSRDSSTDLTQTSSSGTVTLPATIMTSSVPTTVGGHMMYPSPHAVMYAPT SGLGDGSLTVLNAFSQAPSTMQVSHSQVQEPGGVPQVFLTASSGTVQIPVSAVQLHQMAV IGQQAGSSSNLTELQVVNLDTAHSTKSE |
| StorageBuffer | 20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.CurranT.andFranzaB.R.Jr(1988)Cell55,395-397 2.RauscherF.J.D.etal.(1988)Science,240,1010-1016 3.RauscherF.J.D.etal.(1988)Cell52,471-480 4.WagnerE.F.(2001)Oncogene20,2333-2497 5.ChinenovY.andKerppolaT.K.(2001)Oncogene20,2438-2452 6.AngelP.etal.(1987)Cell49,729-739 7.RobinsonM.J.etal.(1998)Curr.Biol.8,1141-1150 |
