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Millipore/14-409 | Histone H2A, Recombinant Xenopus laevis/14-409/100 µg

价格
¥8900.00
货号:14-409
浏览量:127
品牌:Millipore
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商品描述
Description
CatalogueNumber14-409
BrandFamilyUpstate
TradeName
  • Upstate
DescriptionHistoneH2A,RecombinantXenopuslaevis
OverviewRecombinantXenopuslaevisHistoneH2A
AlternateNames
  • H2A
  • HistoneH2A
ProductInformation
StorageandShippingInformation
StorageConditions2yearsat-20C
Applications
ApplicationRecombinantXenopuslaevisHistoneH2A.
KeyApplications
  • EnzymeAssays
BIOLOGicalInformation
SourceproducedinE.coli
SpecificActivityForSpecificActivitydata,refertotheCertificateofAnalysisforindividuallotsofthisenzyme.
EntrezGeneNumber
EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Twomoleculesofeachofthefourcorehistones(H2A,H2B,H3,andH4)formanoctamer,aroundwhichapproximately146bpofDNAiswrappedinrepeatingunits,callednucleosomes.Thelinkerhistone,H1,interactswithlinkerDNAbetweennucleosomesandfunctionsinthecompactionofchromatinintohigherorderstructures.ThisgeneencodesamemberofthehistoneH2Afamily,andgeneratestwotranscriptsthroughtheuseoftheconservedstem-loopterminationmotif,andthepolyAadditionmotif.
GeneSymbol
  • H2AFX
  • H2AX
  • H2a/x
  • H2A/X
  • H2A.X
PurificationMethodHPLC
UniProtNumber
UniProtSummaryFUNCTION:SwissProt:P16104#VarianthistoneH2AwhichreplacesconventionalH2Ainasubsetofnucleosomes.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.Requiredforcheckpoint-mediatedarrestofcellcycleprogressioninresponsetolowdosesofionizingrADIationandforefficientrepairofDNAdoublestrandbreaks(DSBs)specificallywhenmodifiedbyC-terminalphosphorylation.
SIZE:143aminoacids;15145Da
SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.InteractswithnumerousproteinsrequiredforDNAdamagesignalingandrepairwhenphosphorylatedonSer-140.TheseincludeMDC1,TP53BP1,BRCA1andtheMRNcomplex,composedofMRE11A,RAD50,andNBN.InteractionwiththeMRNcomplexismediatedatleastinpartbyNBN.AlsointeractswithDHX9/NDHIIwhenphosphorylatedonSer-140.
SUBCELLULARLOCATION:Nucleus.DEVELOPMENTALSTAGE:SynthesizedinG1aswellasinS-phase.
DOMAIN:SwissProt:P16104The[ST]-QmotifconstitutesarecognitionsequenceforkinasesfromthePI3/PI4-kinasefamily.
PTM:PhosphorylatedonSer-140(toformgamma-H2AFX)inresponsetoDNAdoublestrandbreaks(DSBs)generatedbyexogenousgenotoxicagentsandbystalledreplicationforks,andmayalsooccurduringmeioticrecombinationeventsandimmunoglobulinclassswitchinginlymphocytes.PhosphorylationcanextenduptoseveralthousandnucleosomesfromtheactualsiteoftheDSBandmaymarkthesurroundingchromatinforrecruitmentofproteinsrequiredforDNAdamagesignalingandrepair.Widespreadphosphorylationmayalsoservetoamplifythedamagesignaloraidrepairofpersistentlesions.PhosphorylationofSer-140inresponsetoionizingradiationismediatedbybothATMandPRKDCwhiledefectsinDNAreplicationinduceSer-140phosphorylationsubsequenttoactivationofATRandPRKDC.DephosphorylationofSer-140byPP2AisrequiredforDNADSBrepair.Inmeiosis,Ser-140phosphorylationmayoccuratsynaptonemalcomplexesduringleptoteneasanATM-dependentresponsetotheformationofprogrammedDSBsbySPO11.Ser-140phosphorylationmaysubsequentlyoccursatunsynapsedregionsofbothautosomesandtheXYbivalentduringzygotene,downstreamofATRandBRCA1activation.Ser-140phosphorylationmayalsoberequiredfortranscriptionalrepressionofunsynapsedchromatinandmeioticsexchromosomeinactivation(MSCI),wherebytheXandYchromosomescondenseinpachytenetoformtheheterochromaticXY-body.Duringimmunoglobulinclassswitchrecombinationinlymphocytes,Ser-140phosphorylationmayoccuratsitesofDNA-recombinationsubsequenttoactivationoftheactivation-inducedcytidinedeaminaseAICDA.&MonoubiquitinationofLys-120byRING1andRNF2/RING2complexgivesaspecifictagforepigenetictranscriptionalrepression(Bysimilarity).
SIMILARITY:BelongstothehistoneH2Afamily.
MolecularWeight14kDa
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
Millipore 提供三种不同的 Immobilon PVDF 膜,是针对不同的蛋白质印迹应用的理想选择。 现在我们还 提供采用按规格裁切的印迹方片膜和两层过滤纸组成的印迹三明治。   与硝酸纤维素膜相比,Immobilon 膜具有多种优点。 它们不易破裂或卷 曲,便于裁剪且在切割过程中不会碎裂。 它们还有低背景、广泛溶剂相容性以及优异的着色能力。 此外,它们可以重复检测。 Immobilon-P 膜(沃比森-供应) ·         0.45μm 孔径 ·         建议用于大多数免疫印迹,特别在蛋 白质大于 20kDa 时 ·         Millipore 的快速免疫检测实验方法不需要膜封闭并缩短洗涤时间,将检测时间至少减少了两个小时,而不致降低灵敏 度或特异性 用于高通量实验室的印迹三明治 ·         当您需要处理多个印迹时,印迹三明治是一种便利省时的选择。 我们的印迹三明 治包含多片 Immobilon-P 膜,其中插入了按规格裁切的多张层析级印迹纸。 转印滤纸 ·         按照大多数免疫印迹尺寸进行裁切的层析级转印滤纸。 Immobilon-PSQ 膜(沃比森-供应) ·         0.2μm 孔径和大的内表面面积 ·         较其它膜具有更高的 蛋白质吸附率和测序得率 ·         建议用于小于 20kDa 的印迹蛋白质 ·         防止小分子量的蛋白质的渗漏 Immobilon-FL 膜(沃比森-供应) ·        针对荧光应用进行优化的转印膜 ·         极低的背景提高了所 有荧光检测实验的灵敏度 ·         与所有常用的荧光探针(所有激发和发射波长)兼容 ·         是多态性检测和化学荧光应用的理想选择