Description
Catalogue Number	AB3594-200UL
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Aquaporin 4 Antibody, CT
Alternate Names	Mercurial-Insensitive Water Channel
Background Information	Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special proteins "aquaporins". Aquaporins, are integral membrane proteins from a larger family of major intrinsic proteins (MIP) that form pores in the membrane of biological cells. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. The water-specific channel Aquaporin 4 is located in various tissues including the gastrointestinal tract, kidney and brain. It is an osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. In CNS tissue, AQP4 localizes to astrocyte endfeet making it a useful structural marker.

Product Information
Format	Purified
Control	Included free of charge with the antibody is 120 µg of control antigen (lyophilized powder). Reconstitute with 100 µL of PBS. For positive control, in Western blot use 20 ng of protein per Minigel lane. For negative control, preincubate 3 µg of purified peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation	Purified rabbit polyclonal lyophilized from buffer containing PBS, pH 7.4, containing 1% BSA, and 0.025% sodium azide. Reconstitute with 200 µL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Storage and Shipping Information
Storage Conditions	Stable for up to 6 months at -20ºC from date of receipt. After reconstitution stable at -20ºC in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Applications
Application	Detect Aquaporin 4 using this Anti-Aquaporin 4 Antibody, C-terminus validated for use in IH & WB.
Key Applications	Immunohistochemistry Western Blotting
Application Notes	Immunohistochemistry: A previous lot of this antibody was used on rat brain sections. Dilutions should be made using a carrier protein such as BSA (1-3%). Western blotting: 1:500-1:1,000 using ECL on rat brain membranes. Optimal working dilutions must be determined by the end user

Biological Information
Immunogen	GST fusion protein with residues 249-323 of rat Aquaporin 4 (SwissProt accession number P47863).
Epitope	C-terminus
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes Aquaporin 4 (AQP4, WCH4, Mercurial-Insensitive Water Channel).
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Human, mouse, and rat. Reactivity with other species has not yet been tested. It is expected that the antibody may also react with bovine (71/75) and rabbit (64/75) due to sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_001650.4
Entrez Gene Summary	This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Gene Symbol	AQP4 MIWC WCH4 AQP-4 HMIWC2 MGC22454
Purification Method	ImmunoAffinity Purified
UniProt Number	P55087
UniProt Summary	FUNCTION: SwissProt: P55087 # Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. SIZE: 323 amino acids; 34830 Da SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Brain - muscle > heart, kidney, lung, and trachea. DOMAIN: SwissProt: P55087 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. ...
Molecular Weight	38 kDa

Physicochemical Information

Dimensions

Materials Information

Materials Information

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