Description
CatalogueNumber	MAB2013
BrandFamily	Chemicon®
TradeName	Chemicon
Description	Anti-K-CadherinAntibody,extracellulardomain,clone2B6
AlternateNames	Cadherin-6
BackgroundInformation	CadherinsconstituteafamilyoftransmembraneglycoproteinsinvolvedinCa2+-dependentcell-cellinteractions.Themembersofthisfamilyaredifferentiallyexpressedinvarioustissues.Theyfunctioninthemaintenanceoftissueintegrityandmorphogenesis.Thecadherinsgenerallycontainfiveextracellularrepeats,atransmembranedomainandacytoplasmictailthatbindstothecateninfamilyofcytoskeletalanchoringproteinswhichalsofunctionassignaltransducers.TheextracellulardomainsareresponsIBLeforthespecificityofhomophilicinteractionsbetweencellsexpressingthesamecadherin.CadherinsaredividedintotypeIandtypeIIsubgroups.TypeIcadherinsincludeepithelialcadherin(E-cadherin,cadherin-1oruvomorulin),neuralcadherin(N-cadherinorcadherin-2),placentalcadherin(P-cadherinorcadherin-3)andretinalcadherin(R-cadherinorcadherin-4).Kidneycadherin(K-cadherinorcadherin-6)andosteoblastcadherin(OB-cadherinorcadherin-11)aretypeIIcadherins.Cadherin-6isexpressedintheproximaltubuleepitheliumofthekidneyfromwhichmostrenalcellcarcinomasarederived.

ProductInformation
Format	Purified
Presentation	Purifiedimmuoglobulin.LiquidinPBScontaining0.09%sodiumazide.

StorageandShippingInformation
StorageConditions	1yearat4°Cfromdateofshipment

Applications
Application	Anti-K-CadherinAntibody,extracellulardomain,clone2B6detectslevelofK-Cadherin&hasbeenpublished&validatedforuseinWB,IC,IH.
KeyApplications	WesternBlotting Immunocytochemistry Immunohistochemistry
ApplicationNotes	Westernblot:1:25-1:250 Immunohistochemistry:1:25-1:50;Frozentissuesectionswithavidin-biotinylatedhorserADIshperoxidasecomplex(ABC)asthedetectionmethod. Optimalworkingdilutionsmustbedeterminedbytheenduser.I/

BIOLOGicalInformation
Immunogen	AffinitypurifiedextracellulardomainofhumanK-cadherin
Epitope	extracellulardomain
Clone	2B6
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Mouse
Specificity	ReactswiththeextracellulardomainofK-cadherin.
Isotype	IgG1
SpeciesReactivity	Human Rat
AntibodyType	MonoclonalAntibody
EntrezGeneNumber	NM_004932.2
EntrezGeneSummary	ThisgeneencodesatypeIIclassicalcadherinfromthecadherinsuperfamily.Theencodedmembraneproteinisacalciumdependentcell-celladhesionglycoproteincomprisedoffiveextracellularcadherinrepeats,atransmembraneregionandahighlyconservedcytoplasmictail.Cadherinsmediatecell-cellbindinginahomophilicmanner,contributingtothesortingofheterogeneouscelltypesandthemaintenanceoforderlystructuressuchasepithelium.Strongtranscriptionalexpressionofthisgenehasbeenobservedinhepatocellularandrenalcarcinomacelllines,suggestingapossibleroleinmetastasisandinvasion.
GeneSymbol	CDH6 K-cadherin KCAD
UniProtNumber	P55285
UniProtSummary	FUNCTION:SwissProt:P55285#Cadherinsarecalciumdependentcelladhesionproteins.Theypreferentiallyinteractwiththemselvesinahomophilicmannerinconnectingcells;cadherinsmaythuscontributetothesortingofheterogeneouscelltypes. SIZE:790aminoacids;88309Da SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein. TISSUESPECIFICITY:Highlyexpressedinbrain,cerebellum,andkidney.Lung,pancreas,andgastricmucosashowaweakexpression.Alsoexpressedincertainliverandkidneycarcinomas. SIMILARITY:SwissProt:P55285##Contains5cadherindomains.

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

Millipore 提供三种不同的 Immobilon PVDF 膜，是针对不同的蛋白质印迹应用的理想选择。 现在我们还 提供采用按规格裁切的印迹方片膜和两层过滤纸组成的印迹三明治。   与硝酸纤维素膜相比，Immobilon 膜具有多种优点。 它们不易破裂或卷 曲，便于裁剪且在切割过程中不会碎裂。 它们还有低背景、广泛溶剂相容性以及优异的着色能力。 此外，它们可以重复检测。 Immobilon-P 膜（沃比森-供应） ·         0.45μm 孔径 ·         建议用于大多数免疫印迹，特别在蛋 白质大于 20kDa 时 ·         Millipore 的快速免疫检测实验方法不需要膜封闭并缩短洗涤时间，将检测时间至少减少了两个小时，而不致降低灵敏 度或特异性 用于高通量实验室的印迹三明治 ·         当您需要处理多个印迹时，印迹三明治是一种便利省时的选择。 我们的印迹三明 治包含多片 Immobilon-P 膜，其中插入了按规格裁切的多张层析级印迹纸。 转印滤纸 ·         按照大多数免疫印迹尺寸进行裁切的层析级转印滤纸。 Immobilon-PSQ 膜（沃比森-供应） ·         0.2μm 孔径和大的内表面面积 ·         较其它膜具有更高的 蛋白质吸附率和测序得率 ·         建议用于小于 20kDa 的印迹蛋白质 ·         防止小分子量的蛋白质的渗漏 Immobilon-FL 膜（沃比森-供应） ·        针对荧光应用进行优化的转印膜 ·         极低的背景提高了所 有荧光检测实验的灵敏度 ·         与所有常用的荧光探针（所有激发和发射波长）兼容 ·         是多态性检测和化学荧光应用的理想选择